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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications for the relevant formats include: Blocking the binding between Galectin-9 and Tim-3.
Application References:
1. Fukushima A, et al. 2008. Int Arch Allergy Immunol 146:36
Intracellular staining of mouse thymocytes with RG9-35 PE
Galectin-9 is a mammalian lectin with a molecular weight of 40Kd that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers . It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc). Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or is transported to the cell surface whereby cleavage generates a soluble form. Galectin-9 is involved in events such as cell aggregation, adhesion, chemotaxis and apoptosis, and is important for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
Other Names:
Lgals9, Gal-9, Ecalectin
Structure:
Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs).
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Galectin-9
Intracellular staining of mouse thymocytes with RG9-35 PE
PE anti-mouse Galectin-9
Intracellular staining of mouse thymocytes with the RG9-35 PE
LEAF™ Purified anti-mouse Galectin-9
Intracellular staining of mouse thymocytes with RG9-35 PE
Alexa Fluor® 647 anti-mouse Galectin-9
Intracellular staining of mouse thymocytes with RG9-35 Alexa Fluor® 647
