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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-β1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103808).
Application References:
1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block) 2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block) 3. Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim)) 4. Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block) 5. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) 6. Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC) 7. Cuklerman E, et al. 2001. Science 294:1708. (IHC) 8. Jia W, et al. 2005. Blood 106:3854. (FC)
C57BL/6 mouse bone marrow cells stained with 5H10-27 PE
CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.
Other Names:
α5 integrin, VLA-5 α chain, Integrin α5 chain
Structure:
Integrin family, associates with integrin β1, 135 kD
Distribution:
Thymocytes, activated T cells, mast cells
Function:
Adhesion, costimulation
Ligand Receptor:
Fibronectin
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1994 Blood Cells 20:25. 3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD49e
C57BL/6 mouse bone marrow cells stained with 5H10-27 PE
LEAF™ Purified anti-mouse CD49e
C57BL/6 mouse bone marrow cells stained with 5H10-27 PE
PE anti-mouse CD49e
C57BL/6 mouse bone marrow cells stained with 5H10-27 PE
Purified anti-mouse CD49e
C57BL/6 mouse bone marrow stained with purified 5H10-27, followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD49e
Balb/c mouse bone marrow stained with 5H10-27 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD49e
Balb/c mouse bone marrow stained with 5H10-27 Alexa Fluor® 647
