Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining1,4 of acetone-fixed frozen sections, in vitro stimulation of T cells1 and depletion2 of CD3+ cells in vivo. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100208).
Application References:
1. Miescher, G.C., et al., 1989. Immunol. Lett. 23:113. 2. Mysliwietz, J., et al., 1992. Blood 80:2661. 3. Wu, L., et al., 1991. J. Exp. Med. 174:1617. 4. Zhang, Y., et al., 2002. J. Immunol. 168: 3088. 5. Zan, H., et al., 2005. EMBO J. 24: 3757.
C57BL/6 mouse splenocytes stained with LEAF™ purified 17A2, followed by anti-rat IgG FITC
Description:
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 17A2 antibody recognizes ε/γ (but not ε/δ) of CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo
.
Other Names:
T cell antigen receptor complex, T3
Structure:
Ig superfamily, CD3/TCR, 20 kD
Distribution:
Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function:
Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor:
Peptide antigen/MHC-complex
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Davis, M.M. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss, A. et al., 1994. Cell 76:263.
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