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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, in vitro induction of thymocyte differentiation, and in vivo activation of Treg cells in a mouse model2. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 122004).
Application References:
1. Dennehy KM, et al. 2006. J. Immunol. 176:5725. 2. Beyersdorf N, et al. 2008. Blood online doi:10.1182/blood-2008-03-146662.
C57BL/6 mouse splenocytes stained with LEAF™ E18, followed by anti-mouse IgG2b FITC
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.
Other Names:
Tp44, T44
Structure:
Ig superfamily, 44 kD
Distribution:
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function:
Costimulates T and NK cells
Ligand Receptor:
CD80 (B7-1), CD86 (B7-2)
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD28
C57BL/6 mouse splenocytes stained with purified E18, followed by anti-mouse IgG2b FITC
LEAF™ Purified anti-mouse CD28
C57BL/6 mouse splenocytes stained with LEAF™ E18, followed by anti-mouse IgG2b FITC
FITC anti-mouse CD28
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and E18 FITC
PE anti-mouse CD28
C57BL/6 mouse splenocytes stained with E18 PE
PE/Cy7 anti-mouse CD28
C57BL/6 mouse thymocytes stained with E18 PE/Cy7
APC anti-mouse CD28
C57BL/6 mouse thymocytes stained with E18 APC
Alexa Fluor® 488 anti-mouse CD28
C57BL/6 mouse splenocytes stained with E18 Alexa Fluor® 488
