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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) 2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) 3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
ConA-activated C57BL/6 mouse splenocytes (3 days) stained with LEAF™ purified C9B7W, followed by anti-rat IgG FITC
CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
Other Names:
LAG3, LAG-3
Structure:
Ig superfamily
Distribution:
T cells and NK cells, as well as T regulatory cells
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
LEAF™ Purified anti-mouse CD223 (LAG-3)
ConA-activated C57BL/6 mouse splenocytes (3 days) stained with LEAF™ purified C9B7W, followed by anti-rat IgG FITC
Biotin anti-mouse CD223 (LAG-3)
ConA-stimulated (3days) C57BL/6 splenocytes were stained with biotinylated CD223 (clone C9B7W) (filled histogram) or rat IgG1 isotype control (open histogram), followed by Sav-PE.
PE anti-mouse CD223 (LAG-3)
ConA-stimulated C57BL/6 splenocytes stained with C9B7W PE
APC anti-mouse CD223 (LAG-3)
Con A-stimulated (day-3) C57BL/6 splenocytes stained with C9B7W APC
