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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, and costimulation (synergistic with anti-IgM) of B cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 115514).
Application References:
1. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 2. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) 3. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) 4. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) 5. Andoniou CE, et al. 2005. Nature Immunol. 6:1011. (FC) 6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) 7. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) 8. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. PubMed 9. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 10. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
Other Names:
B4
Structure:
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution:
Pro-B to mature B (during development), follicular dendritic cells
Function:
Modulates B cell activation and differentiation
Ligand Receptor:
CD21, CD81, Leu-13
Antigen References:
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD19
C57BL/6 mouse splenocytes stained with CD19 (clone 6D5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Biotin anti-mouse CD19
C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
FITC anti-mouse CD19
C57BL/6 splenocytes were stained with CD19 (clone 6D5) FITC and CD3 PE.
LEAF™ Purified anti-mouse CD19
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
PE anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
PE/Cy5 anti-mouse CD19
C57BL/6 splenocytes were stained with CD19 (clone 6D5) PE/Cy5 and CD3 FITC.
Purified anti-mouse CD19
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
PE/Cy7 anti-mouse CD19
C57BL/6 splenocytes were stained with CD19 (clone 6D5) PE/Cy7 and CD3 FITC.
Alexa Fluor® 488 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
Alexa Fluor® 647 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Alexa Fluor® 647 (filled histogram) or rat IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
Pacific Blue™ anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Pacific Blue™ (filled histogram) or rat IgG2a, κ Pacific Blue™ isotype control (open histogram).
Alexa Fluor® 700 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
APC/Cy7 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) APC/Cy7 (filled histogram) or rat IgG2a, κ APC/Cy7 isotype control (open histogram).
PerCP anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PerCP (filled histogram) or rat IgG2a, κ PerCP isotype control (open histogram).
PerCP/Cy5.5 anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PerCP/Cy5.5 (filled histogram) or rat IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).
Brilliant Violet 421™ anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-mouse CD19
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD19 (clone 6D5) Brilliant Violet 570™ (top) or rat IgG2a, κ Brilliant Violet 570™ isotype control (bottom).
