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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8,immunofluorescence microscopy6,7,10 and immunohistochemistry of acetone-fixed frozen sections5,11-13 and paraffin sections28.
Application References:
1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP) 2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete) 3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block) 4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP) 5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC) 6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF) 7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF) 8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete) 9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block) 10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF) 11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC) 13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC) 14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC) 15. Olver S, et al. 2006. Cancer Research 66:571. (FC) 16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed 17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC) 18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) 19. Fazilleau N, et al. 2007. Nature Immunol. 8:753. 20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed 21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed 22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed 23. Kim DD, et al. 2008. Blood 112:1109. PubMed 24. Guo Y, et al. 2008. Blood 112:480. PubMed 25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed 26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed 27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
C57BL/6 mouse bone marrow cells were stained with LEAF™ purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Other Names:
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR
Structure:
Integrin family, associates with integrin ß2 (CD18), 170 kD
Distribution:
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram) (gated on total cells).
Biotin anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with biotinylated CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE (gated on total cells).
FITC anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) FITC (top) or rat IgG2b, κ FITC isotype control (bottom) (gated on total viable cells).
LEAF™ Purified anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with LEAF™ purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
PE anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram) (gated on total cells).
PE/Cy5 anti-mouse CD11b
C57BL/6 mouse bone marrow cells stained with CD11b (clone M1/70) PE/Cy5 (filled histogram) or rat IgG2b, κ PE/Cy5 isotype control (open histogram) (gated on total cells).
Purified anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
PE/Cy7 anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE/Cy7 (filled histogram) or rat IgG2b, κ PE/Cy7 isotype control (open histogram) (gated on total cells).
Alexa Fluor® 488 anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 488 (filled histogram) or rat IgG2b Alexa Fluor® 488 isotype control (open histogram) (gated on total cell population).
Alexa Fluor® 647 anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 647 or rat IgG2b, κ Alexa Fluor® 647 isotype control (gated on total cells).
Alexa Fluor® 700 anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram) (gated on total cells).
Pacific Blue™ anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram) (gated on total cells).
APC/Cy7 anti-mouse CD11b
C57BL/6 mouse bone marrow stained with M1/70 APC/Cy7
PerCP/Cy5.5 anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram) (gated on total cells).
PerCP anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP (filled histogram) or rat IgG2b PerCP isotype control (open histogram) (gated on total cell population).
Brilliant Violet 421™ anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 570™ anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 570™ (filled histogram) or rat IgG2b, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 605™ anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 605™. Data shown was gated on myeloid cell population.
Brilliant Violet 650™ anti-mouse CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 650™. Data shown was gated on myeloid cell population.
