Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2, and immunohistochemical staining3 of acetone-fixed frozen sections. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101110).
Application References:
1. Sanchez-Madrid, F., et al., 1982. Cell Immunol. 73:1. 2. Kuhlman, P., et al., 1991. J. Immunol. 146:1773. 3. Mizgerd, J. P., et al., 1997. J. Exp. Med. 186:1357. 4. Hailman, E. and Allen, P. M., 2005. J. Immunol. 175:4847. 5. Morley, SC., et al. 2010. J. Immunol. 184:3628. PubMed.
C57BL/6 mouse splenocytes stained with LEAF™ purified M17/4, followed by anti-rat IgGs FITC
Description:
CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The M17/4 antibody can block CD11a-mediated cellular adhesion.
Other Names:
αL integrin, LFA-1 α subunit, Ly-15, Ly-21
Structure:
Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution:
Lymphocytes, granulocytes, monocytes, macrophages
Function:
Cellular adhesion
Ligand Receptor:
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Springer, T.A. 1994. Cell 76:301. 3. Lub, M., et al., 1995. Immunol. Today 16:479.
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