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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
CXCR7 transfected mouse L1.2 cells stained with LEAF™ purified 8F11-M16, followed by anti-mouse IgG PE
CXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR). CXCR7 binds with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. Binding of ligand to CXCR7 induces proliferation and migration of immature neurons, glia and their precursors. CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. Surface expression of CXCR7 was also reported to be on tumor cells, activated endothelial cells, fetal liver cells, and few other cell types.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human/mouse CXCR7
CXCR7 transfected mouse L1.2 cells stained with purified 8F11-M16, followed by anti-mouse IgG PE
LEAF™ Purified anti-human/mouse CXCR7
CXCR7 transfected mouse L1.2 cells stained with LEAF™ purified 8F11-M16, followed by anti-mouse IgG PE
PE anti-human/mouse CXCR7
CXCR7 transfected mouse L1.2 cells stained with 8F11-M16 PE
Biotin anti-human/mouse CXCR7
CXCR7 transfected mouse L1.2 cells stained with biotinylated 8F11-M16, followed by Sav-PE
APC anti-human/mouse CXCR7
CXCR7 transfected L1.2 cells were stained with CXCR7 (clone 8F11-M16) APC (filled histogram) or mouse IgG2b APC isotype control (open histogram).
