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LEAF™ Purified anti-human IL-1β Antibody
LEAF™ Purified anti-human IL-1β Antibody
511604 500 µg ¥60,000     

Product Details

Clone: H1b-27
Isotype: Mouse IgG2b, κ
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:

ELISA Capture-Quality tested
ELISPOT Capture, Block - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Description:

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. 

Other Names: Interleukin-1β, H1, OAF, Catabolin, IFNb-inducing factor(il-1b,il1b)
Structure: Cytokine; 17.3 kD
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Cellular Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cellular Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Bioactivity/Activities: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Antigen
References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

GeneID: 3553
Latest Publications: View the latest IL-1beta articles on HighwirePress.com
UniProt: View information about IL-1beta on UniProt.org
Keywords: LEAF™ Purified anti-human IL-1β, H1b-27, LEAF™, Interleukin-1β, H1, OAF, Catabolin, IFNb-inducing factor(il-1b,il1b), Human, Immunology, Antibodies
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