Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by ELISA analysis. For ELISA applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA or ELISPOT Capture1,2: The Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The LEAF™ Purified antibody is suggested for ELISPOT capture.
Application References:
1. McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18. 2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20. 3. Schaerli, P., et al. 2000. J. Exp. Med. 192:1553. 4. Andersson, J., et al. 1994. Immunology 83:16. 5. Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916. 6. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. 7. Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577. 8. Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13.
Other Names:
Interleukin-13, NC30
Structure:
Cytokine; 17 kD (Mammalian)
Regulation:
IL-13Rα2 possible antagonist
Cellular Sources:
Activated T cells, mast cells, NK cells
Cellular Targets:
B cells, monocytes, macrophages, basophils
Receptors:
Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Bioactivity/Activities:
Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497. 3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797. 4. Ochensberger, B., et al. 1996. Blood 88:3028.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human IL-13
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with APC rat IgG1,k isotype control (top) or APC JES10-5A2 (bottom)
LEAF™ Purified anti-human IL-13
PE anti-human IL-13
PMA+ionomycin-stimulated T lymphocytes intracellularly stained with JES10-5A2 PE and CD4 (RPA-T4) APC
Purified anti-human IL-13
PerCP/Cy5.5 anti-human IL-13
PMA-restimulated Th2 polarized human peripheral blood lymphocytes intracellular stained with CD3 FITC and JES10-5A2 PerCP/Cy5.5
