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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4° C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA or ELISPOT Capture1,2: The Purified BVD13-3A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD11-37G10 antibody (Cat. No. 506702) as the detecting antibody. The LEAF™ Purified antibody is suggested for ELISPOT capture.
Application References:
1. Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20. 2. Abrams J, et al. 1992. Immunol. Rev. 127:5. 3. Andersson J, et al. 1994. Immunology 83:16. 4. Sander B, et al. 1991. Immunol. Rev. 119:65. 5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma. The BVD13-3A5 antibody reacts with human granulocyte-colony stimulating factor (G-CSF). The BVD13-3A5 antibody can neutralize the bioactivity of natural or recombinant G-CSF.
Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2
Cellular Sources:
Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells
Cellular Targets:
Hematopoietic progenitors of neutrophil/granulocyte lineage, endothelial cells
Receptors:
CD114 (G-CSFR)
Bioactivity/Activities:
Growth/differentiation/activating factor for neutrophils; proliferation/migration endothelial cells cells; synergistic with IL-3 growth of hematopoietic progenitors
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Demetri G, et al. 1991. Blood 78:2791. 3. Moore M. 1991. Annu. Rev. Immunol. 9:159. 4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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