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LEAF™ Purified anti-human Delta-like protein 4 (DLL4) Antibody
LEAF™ Purified anti-human Delta-like protein 4 (DLL4) Antibody
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications for the relevant formats include: blocking of DLL4 binding to Notch 1 and Notch 4.
Application References:
1. Yamanda S, et al. 2009. Blood 113:3631.
Description:
Delta-like protein 4 (DLL4) is a type I transmembrane protein whose extracellular domain contains a DSL domain and eight tandem EGF repeats. After binding of its ligands Notch 1 and Notch 4, the extracellular and cytoplasmic domains are cleaved and the cytoplasmic domain is translocated into the nucleus. DLL4 is expressed in the vascular endothelium, neural epithelium, adrenal cortex, spleen, and lymph nodes. It is also highly expressed in some types of bladder, breast, and renal carcinomas. DLL4 is critical in vascular development and homeostasis.
Other Names:
Delta4, Drosophila Delta homolog 4
Structure:
Type I transmembrane protein whose extracellular domain contains a DSL domain and eight tandem EGF repeats
Distribution:
Vascular endothelium, neural epithelium, adrenal cortex, spleen, and lymph nodes; highly expressed in some types of bladder, breast and renal carcinomas
Function:
Vascular development and homeostasis
Ligand Receptor:
Notch 1 and Notch 4
Antigen References:
1. Beck RC, et al. 2009. Biol Blood Marrow Transplant. 15:1026. 2. Mazella J, et al. 2008. Endocrinology. 149:15. 3. Noguera-Troise I, et al. 2006. Nature 444:1032. 4. Sugimoto A, et al. 2006. Exp Hematol. 34:424. 5. Liu ZJ, et al. 2003. Mol Cell Biol. 23:14.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
