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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation5, immunohistochemical staining of acetone-fixed frozen tissue sections5, immunofluorescence microscopy5, and blocking of cell adhesion4,6. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 304414).
Application References:
1. Knapp WB, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. 2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 3. Horton M, et al. 1991. Exp. Cell Res. 195:368. 4. Takahashi R, et al. 1999. Blood 93:1951. (Block) 5. Davies J, et al. 1989. J. Cell Biol. 109:1817. (IF, IHC, IP) 6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. (Block) 7. Barau A, et al. 2010. J. Ultrasound Med. 29:173. PubMed
Human melanoma cell line M21 stained with LEAF™ purified 23C6, followed by anti-mouse IgG FITC
CD51/CD61 is an integrin complex known as αVβ3. It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or β3 integrin. It is a 110 kD common β subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. The 23C6 antibody has been reported to be useful for blocking studies.
Other Names:
Vitronectin receptor, Integrin αV/β3
Structure:
Integrin, CD51/CD61 heterodimer, 24 kD
Distribution:
Platelets, B subset, activated T cells, monocytes/macrophages, osteoblasts, endothelial cells, melanoma
Function:
Adhesion, bone metabolism and apoptosis
Ligand Receptor:
Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen
Antigen References:
1. Davies J, et al. 1989. J. Cell Biol. 109:1817. 2. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD51/61
Human melanoma cell line M21 stained with 23C6 FITC
PE anti-human CD51/61
Human melanoma cell line M21 stained with 23C6 PE
Purified anti-human CD51/61
Human melanoma cell line M21 stained with purified 23C6, followed by anti-mouse IgGs FITC
Alexa Fluor® 488 anti-human CD51/61
Human melanoma cell line M21 stained with 23C6 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD51/61
Human melanoma cell line M21 stained with 23C6 Alexa Fluor® 647
Biotin anti-human CD51/61
Human melanoma cell line M21 stained with biotinylated 23C6, followed by Sav-PE
LEAF™ Purified anti-human CD51/61
Human melanoma cell line M21 stained with LEAF™ purified 23C6, followed by anti-mouse IgG FITC
