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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of α5 integrin mediated cell adhesion. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 328004).
Application References:
1. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with LEAF™ purified NKI-SAM-1, followed by anti-mouse IgG FITC
CD49e is a type I integral membrane glycoprotein, known as α5 integrin, VLA-5 α chain. It forms noncovalent heterodimer with integrin β1 (CD29). CD49e contains two disulfide-linked subunits of 135 kD and 24 kD, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.
Other Names:
α5 integrin, VLA-5 α chain, Integrin α5 chain
Structure:
Integrin family, associates with integrin ß1 (CD29), 135 kD and 25 kD in reduced condition, 155 kD (unreduced)
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD49e
Human peripheral blood lymphocytes stained with purified NKI-SAM1, followed by anti-mouse IgG FITC
LEAF™ Purified anti-human CD49e
Human peripheral blood lymphocytes stained with LEAF™ purified NKI-SAM-1, followed by anti-mouse IgG FITC
Biotin anti-human CD49e
Human peripheral blood lymphocytes stained with biotinylated NKI-SAM-1, followed by Sav-PE
FITC anti-human CD49e
Human peripheral blood lymphocytes stained with NKI-SAM-1 FITC
PE anti-human CD49e
Human peripheral blood lymphocytes stained with NKI-Sam-1 PE
APC anti-human CD49e
Human peripheral blood lymphocytes stained with NKI-SAM1 APC
