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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) CD3 antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The CD3 antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. 3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. 4. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed 5. Alter G, et al. 2008. J. Virol. 82:9668. PubMed 6. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed 7. Biggs MJ, et al. 2011. J R Soc Interface. PubMed
Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Other Names:
T3, CD3ε
Structure:
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution:
Mature T and NK-T cells, thymocyte differentiation
Function:
Antigen recognition, signal transduction, T cell activation
Ligand Receptor:
Peptide antigen bound to MHC
Antigen References:
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD3
Human peripheral blood lymphocytes stained with purified OKT3, followed by anti-mouse IgGs-FITC
LEAF™ Purified anti-human CD3
Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC
FITC anti-human CD3
Human peripheral blood lymphocytes stained with OKT3 FITC
PE anti-human CD3
Human peripheral blood lymphocytes stained with OKT3 PE
Alexa Fluor® 488 anti-human CD3
Human peripheral blood lymphocytes stained with OKT3 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD3
Human peripheral blood lymphocytes stained with OKT3 Alexa Fluor® 647
APC anti-human CD3
Human peripheral blood lymphocytes stained with OKT3 APC
Biotin anti-human CD3
Human peripheral lymphocytes were stained with biotinylated CD3 (clone OKT3) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by Sav-PE.
Brilliant Violet 605™ anti-human CD3
Human peripheral lymphocytes were stained with CD3 (clone OKT3) Brilliant Violet 605™ (filled histogram).
Brilliant Violet 650™ anti-human CD3
Human peripheral lymphocytes were stained with CD3 (clone OKT3) Brilliant Violet 650™.
Ultra-LEAF™ Purified anti-human CD3
Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC
