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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining3,5 of acetone-fixed frozen tissue sections, and activation4,7,8 of integrin β1. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 303010).
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC) 2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208. (FC) 3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (FC IP IHC) 4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ) 5. Frank NY, et al. 2005. Cancer Res. 65:4320. (FC IHC) 6. Murga M, et al. 2005. Blood 105:1992. (FC) 7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ) 8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ) 9. Barbolina MV, et al. 2010. Mol Cancer Res. 8:653. PubMed
Human peripheral blood lymphocytes stained with LEAF™ purified TS2/16, followed by anti-mouse IgGs FITC
CD29 is a 130 kD single chain type I glycoprotein, known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins which include CD29 bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.
Other Names:
Integrin β1 chain, VLA-β chain, gpIIa
Structure:
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 APC
PE anti-human CD29
Human peripheral blood lymphocytes stained
with TS2/16 PE
PE/Cy5 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 PE/Cy5
Purified anti-human CD29
Human peripheral blood lymphocytes stained with purified TS2/16, followed by anti-mouse IgGs FITC
LEAF™ Purified anti-human CD29
Human peripheral blood lymphocytes stained with LEAF™ purified TS2/16, followed by anti-mouse IgGs FITC
APC/Cy7 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 APC/Cy7
Alexa Fluor® 488 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 Alexa Fluor® 700
