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Human, Cross-Reactivity: Chimpanzee, Common Marmoset, Squirrel Monkey
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA) 2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA) 3. Velu V, et al. 2007. J. Virol. 81:5819. 4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed 5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed 6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA) 7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed 8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed 9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed 10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed 11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
Description:
The program death 1 (PD-1) receptor CD279 is a 55 kD member of the immunoglobulin superfamily. CD279/PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279/PD-1 is expressed predominantly on activated T cells, B cells and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279/PD-1 and are members of the B7 gene family. Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279:PD-ligands results in inhibition of T cell proliferation and cytokine secretion.
Other Names:
PD-1
Structure:
Immunoglobulin superfamily
Distribution:
Transiently expressed on CD4- CD8- thymocytes; stimulated thymocytes and splenic T and B lymphocytes; activated myeloid cells
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