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300.19 cell line transfected with the CD229 full-length DNA
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, and immunocytochemistry3.The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (Cat. No. 326104).
Application References:
1. de la Fuente MA, et al. 2001. Blood 97:3513. 2. Martin M, et al. 2005. J. Immunol. 174:5977. 3. Romero X, et al. 2005. J. Immunol. 174:7033.
Description:
CD229 is a 100-120 kD glycoprotein. It is a member of the SLAM family, a CD2 subset of the Ig superfamily, known as Ly9 or SLAMF3. CD229 is expressed on T cells, B cells, NK cells, and thymocytes. It functions as a homophilic adhesion molecule through binding to CD229 itself. The cytoplasmic tail of CD229 binds to SAP and Grb2 proteins. CD229 is involved in enhancing T cell activation and Th2 polarization. HLy9.1.25 antibody recognizes epitope A in the first Ig domain of Ly9 molecule.
Other Names:
Ly9, Ly-9, SLAMF3
Structure:
100-120 kD glycoprotein, SLAM family, CD2 subset of Ig superfamily
Distribution:
T cells, B cells, NK cells, thymocytes
Function:
Homophilic adhesion, T cell activation, Th2 cell polarization
Ligand Receptor:
CD229
Antigen References:
1. Sandrin MS, et al. 1996. Immunogenetics 43:13. 2. de la Fuente MA, et al. 2001. Blood 97:3513. 3. Romero X, et al. 2005. J. Immunol. 174:7033.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
