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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking1-3 of hIL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308806).
Application References:
1. Liu Y, et al. 1997. J. Immunol. 158:604. 2. Levings MK, et al. 2005. Blood 105:1162. 3. Goodier MR, et al. 2000. J. Immunol. 165:139. 4. Huang YH, et al. 2009. J. Leukoc. Biol. 86:273. PubMed 5. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with LEAF™ purified 3F9, followed by biotinylated anti-rat IgG and Sav-PE
CD210, also known as the IL-10 receptor, is a 90-110 kD protein expressed on T cells, B cells, NK cells, monocytes and macrophages. CD210 belongs to the class II cytokine receptor family which includes the IFN-γ receptor (CDw119), the IFN-α/β receptor (CD118) and tissue factor (CD142). The IL-10 receptor is involved in signal transduction by inducing phosphorylation of STAT1a and STAT3 and by inducing activation of Jak1 and Tyk.
Other Names:
IL-10 Receptor, IL-10R
Structure:
Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD
Distribution:
T and B cells, NK cells, monocytes, macrophages
Function:
Activates STAT1, STAT3, Jak1, Tyk
Ligand Receptor:
IL-10
Antigen References:
1. Kotenko S. 2002. Cytokine Growth Factor Rev. 13:223. 2. Trinchieri G. 2003. Nat. Rev. Immunol. 3:133.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
LEAF™ Purified anti-human CD210 (IL-10 R)
Human peripheral blood lymphocytes stained with LEAF™ purified 3F9, followed by biotinylated anti-rat IgG and Sav-PE
PE anti-human CD210 (IL-10 R)
Human peripheral blood lymphocytes stained with 3F9 PE
Purified anti-human CD210 (IL-10 R)
Whole blood lymphocytes stained
with purified 3F9, then detected
with biotinylated anti-rat IgG
followed by Sav-PE
