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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Intracellular staining is recommended to detect KIR2DL4 in NK cells, as this receptor resides predominantly in endosomes3. Alternatively, mAb 33 can be loaded into NK cells by endocytosis at 37°C3. This antibody also induces NK cell cytotoxicity1,2.
Application References:
1. Rajagopalan S, et al. 2001. J. Immunol. 167:1877. (FC) 2. Goodridge JP, et al. 2003. J. Immunol. 171:1768. (FC) 3. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
Human peripheral blood lymphocytes were cultured 16 hrs with IL-2, then stained with CD56 (clone HCD56) APC and CD158d (clone 33) PE (top) or mouse IgG1, κ PE isotype control (bottom).
CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs, with long cytoplasmic domains, contain ITIMs and possess inhibitory functions and others, with short cytoplasmic regions, lack ITIM and have activation functions. Fourteen polymorphic KIR genes have been reported in humans. KIR2DL4 (CD158d) is a unique receptor which has an ITIM in its cytoplasmic domain and a charged residue in the transmembrane domain. It possesses both inhibitory and activation functions. Two common alleles (10A and 9A) of KIR2DL4 have been reported. The 10A allele (with 10 adenines at the end of the transmembrane exon) receptor is expressed on CD56high NK subset, whereas its expression on CD56dim NK cells is inducible upon culture. The major 9A allele receptor is a secreted form. HLA-G is the ligand of CD158d.
Other Names:
KIR2DL4, 2DL4, p49, KIR103
Structure:
Contains two Ig-like extracellular domains, 45-50 kD
Distribution:
On CD56high NK cells and cultured CD56dim NK cells
Ligand Receptor:
HLA-G
Antigen References:
1. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9. 2. Goodridge JP, et al. 2007. Eur. J. Immunol. 37:199.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD158d (KIR2DL4)
Human peripheral blood lymphocytes were cultured 16 hrs with IL-2, then stained with CD56 (clone HCD56) APC and CD158d (clone 33) PE (top) or mouse IgG1, κ PE isotype control (bottom).
LEAF™ Purified anti-human CD158d (KIR2DL4)
Human peripheral blood lymphocytes were cultured 16 hrs with IL-2, then stained with CD56 (clone HCD56) APC and CD158d (clone 33) PE (top) or mouse IgG1, κ PE isotype control (bottom).
PE anti-human CD158d (KIR2DL4)
Human peripheral blood lymphocytes were cultured 16 hrs with IL-2, then stained with CD56 (clone HCD56) APC and CD158d (clone 33) PE (top) or mouse IgG1, κ PE isotype control (bottom).
APC anti-human CD158d (KIR2DL4)
IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
