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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) CD154 antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The CD154 antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF) 2. Rushworth SA, et al. 2002. Transplantation 73:635. (Block) 3. Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF) 4. Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block) 5. Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed 6. Kuchen S, et al. 2007. J. Immunol. 179:5886. 7. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with CD154 (clone 24-31) PE
CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.
Other Names:
CD40L, gp39, TRAP, T-BAM, TNFSF5
Structure:
TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution:
Activated T cells
Function:
B cell costimulation
Ligand Receptor:
CD40
Antigen References:
1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227. 2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275. 3. Ford G, et al. 1999. J. Immunol. 162:4037.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD154
PMA + ionomycin-stimulated human PBMCs (5 hours) stained with purified 24-31, followed by anti-mouse IgG FITC
FITC anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (6 hours) stained with 24-31 FITC
PE anti-human CD154
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with 24-31 PE
PE/Cy5 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (6 hours) stained with 24-31 PE/Cy5
APC anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with 24-31 APC
LEAF™ Purified anti-human CD154
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with CD154 (clone 24-31) PE
Biotin anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with biotinylated 24-31, followed by Sav-PE
Alexa Fluor® 488 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with 24-31 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with 24-31 Alexa Fluor® 647
Pacific Blue™ anti-human CD154
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with 24-31 Pacific Blue™
APC/Cy7 anti-human CD154
TPA+ ionomycin-stimulated human PBMCs (5 hours) stained with 24-31 APC/Cy7
Brilliant Violet 421™ anti-human CD154
PMA+ionomycin-stimulated human peripheral blood lymphocytes were stained with CD154 (clone 24-31) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 605™ anti-human CD154
6-hour PMA+ionomycin-stimulated human peripheral blood lymphocytes were stained with CD154 (clone 24-31) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).
Ultra-LEAF™ Purified anti-human CD154
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with CD154 (clone 24-31) PE
