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Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein
Formulation:
Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion1,2.
Application References:
1. May K, et al. 2005. Blood 105:1114. (Block) 2. Lute K, et al. 2005. Blood 106:3127. (Block)
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with purified L3D10 conjugated with PE and CD3 APC
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control
CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. Regulatory T cells express high level of CTLA-4. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation.
Other Names:
Cytotoxic T-Lymphocyte Antigen 4
Structure:
Ig superfamily, 33 kD
Distribution:
Activated T and B cells
Function:
Negative regulator of T cell activation
Ligand Receptor:
B7-1 (CD80), B7-2 (CD86)
Antigen References:
1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego. 2. Kuiper H, et al. 1995. J. Immunol. 155:1776. 3. Lindsten T, et al. 1993. J. Immunol. 151:3489 4. Morton P, et al. 1996. J. Immunol. 156:1047.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD152 (CTLA-4)
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with purified L3D10 conjugated with PE and CD3 APC
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control
PE anti-human CD152 (CTLA-4)
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with L3D10 PE and CD3 APC
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control
LEAF™ Purified anti-human CD152 (CTLA-4)
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with purified L3D10 conjugated with PE and CD3 APC
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control
APC anti-human CD152 (CTLA-4)
PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD3 FITC and CD152 (clone L3D10) APC (top) or mouse IgG1 APC isotype control (bottom).
