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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of T cell activation2, immunohistochemical staining1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting1. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 306206).
Application References:
1. Menashi S, et al. 2003. Cancer Res. 63:7575. (WB IHC) 2. Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block) 3. Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)
Human peripheral blood lymphocytes stained with LEAF™ purified HIM6, followed
by anti-mouse IgG FITC
CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.
Other Names:
Neurothelin, Basigin, EMMPRIN
Structure:
Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD147
Human peripheral blood lymphocytes stained with HIM6 FITC
Purified anti-human CD147
Human peripheral blood lymphocytes stained with purified HIM6, followed
by anti-mouse IgG FITC
LEAF™ Purified anti-human CD147
Human peripheral blood lymphocytes stained with LEAF™ purified HIM6, followed
by anti-mouse IgG FITC
Alexa Fluor® 488 anti-human CD147
Human peripheral blood lymphocytes stained with HIM6 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD147
Human peripheral blood lymphocytes stained with HIM6 Alexa Fluor® 647
