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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.
Preparation:
The LEAF™ (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Concentration:
1.0 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications include: inhibit CD11c mediated adhesion, stimulate chemokine production by monocytes, Bu15 binding epitope is different from mAb 3.9 (another CD11c clone), the binding of mAb Bu15 with CD11c is divalent cation independent
Application References:
1. Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. 2. Rezzonico R, et al. 2001. Blood 97:2932. 3. Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395. 4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood granulocytes stained with LEAF™ purified Bu15, followed by anti-mouse IgG FITC
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αx, and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
Other Names:
Integrin αx subunit, CR4, p150 Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution:
Myeloid, dendritic cells, NK cells, B and T cell subsets
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC/Cy7 anti-human CD11c
Human peripheral blood monocytes stained with BU15 APC/Cy7
Purified anti-human CD11c
Human peripheral blood granulocytes stained with purified Bu15, followed by anti-mouse IgG FITC
LEAF™ Purified anti-human CD11c
Human peripheral blood granulocytes stained with LEAF™ purified Bu15, followed by anti-mouse IgG FITC
PE anti-human CD11c
Human peripheral blood lymphocytes stained with Bu15 PE
APC anti-human CD11c
Human peripheral blood lymphocytes stained with Bu15 APC
PerCP/Cy5.5 anti-human CD11c
Human peripheral blood granulocytes stained with Bu15 PerCP/Cy5.5
Pacific Blue™ anti-human CD11c
Human peripheral blood granulocytes stained with Bu15 Pacific Blue™
FITC anti-human CD11c
Human peripheral blood monocytes stained with BU15 FITC.
PE/Cy7 anti-human CD11c
Human peripheral blood monocytes stained with Bu15 PE/Cy7
