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Instrument Guide
Welcome to BioLegend’s Flow Cytometry Instrument Guide/Fluorophore Selector tool. Here, we provide a general view of which fluorophores can be used on commercially available instruments. As instrument configurations and laser options can vary widely, be sure to check with your own instrument to determine if the configuration matches those presented here or if your instrument can be modified/configured to accommodate certain fluorophores.
  Exmax Emmax
Alexa Fluor® 405 401 421
Brilliant Violet 421™ 405 421
Pacific Blue™ 410 455
BD Horizon™ V450 406 450
Brilliant Violet 510™ 405 510
BD Horizon™ V500 415 500
AmCyan 458 489
Pacific Orange™ 400 551
Brilliant Violet 570™ 405 570
Brilliant Violet 605™ 405 603
Brilliant Violet 650™ 405 645
Brilliant Violet 711™ 405 711
Brilliant Violet 785™ 405 785
Alexa Fluor® 488 495 518
FITC (fluorescein) 493 525
PE (R-Phycoerythrin) 565 575
Cy3 514 566
PE/Cy5 565 670
PerCP 482 675
PerCP/Cy5.5 482 690
PE/CF594 565 610
PE/Dazzle™ 594 565 610
PE/Cy7 565 774
APC (allophycocyanin) 645 660
Alexa Fluor® 647 650 668
Alexa Fluor® 700 696 719
APC/Cy7 650 774
APC/Alexa Fluor® 750 650 774
Hoechst 33342 350 450
DAPI 359 461
7-AAD 546 647
PI (Propidium Iodide) 540 620
To compare instruments, press and hold the Control key to make multiple selections, selecting all your desired manufacturers first, followed by desired instruments and lastly, the laser options for each instrument.
Laser Guide:
UV = Ultraviolet (350-365 nm)
V = Violet (405-407 nm)
B = Blue (488 nm)
G = Green (532 nm)
Y = Yellow (561 nm)
R = Red (633-640 nm)

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