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Cell Adhesion

Cell adhesion molecules are necessary for many different cellular functions including cell to cell signaling, cell migration, cell rolling, activation, cell killing, apoptosis, maturation, formation of tight junctions, as well as other functions. Understanding cell adhesion is important in fully elucidating immune cell functions. Biolegend provides an array of antibodies against adhesion molecules for the study of cell adhesion, including applications such functional assays, western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.

1.View Our Cell Adhesion Molecule antibodies

Human Cell Adhesion Molecules

Mouse Cell Adhesion Molecules.

2.Download our Interactive Human and Mouse CD Molecule Charts

Interactive Human CD Molecule chart

Interactive Mouse CD Molecule chart

3. View recent Cell Adhesion articles using Biolegend products.

1. NLR family member NLRC5 is a transcriptional regulator of MHC class I genes. Torsten B. Meissner, Amy Li, Amlan Biswas, Kyoung-Hee Lee, Yuen-Joyce Liu, Erkan Bayir, Dimitrios Iliopoulos, Peter J. van den Elsen, and Koichi S. Kobayashi. PNAS. Aug 2010; 107: 13794 - 13799. PubMed.
2. Rolling on E- or P-selectin induces the extended but not high-affinity conformation of LFA-1 in neutrophils. Yoshihiro Kuwano, Oliver Spelten, Hong Zhang, Klaus Ley, and Alexander Zarbock. Blood. Jul 2010; 116: 617 - 624. PubMed.
3. EDA-containing cellular fibronectin induces fibroblast differentiation through binding to 47 integrin receptor and MAPK/Erk 1/2-dependent signaling. Martin Kohan, Andres F. Muro, Eric S. White, and Neville Berkman. FASEB J. Jul 2010; 10.1096/fj.10-154435. PubMed.
4. β1 Integrin Expression Increases Susceptibility of Memory B Cells to Epstein-Barr Virus Infection. Marcus Dorner, Franziska Zucol, Davide Alessi, Stephan K. Haerle, Walter Bossart, Markus Weber, Rahel Byland, Michele Bernasconi, Christoph Berger, Sharof Tugizov, Roberto F. Speck, and David Nadal. J. Virol. Jul 2010; 84: 6667 - 6677. PubMed.
5. α4β1 Integrin Mediates the Recruitment of Immature Dendritic Cells across the Blood-Brain Barrier during Experimental Autoimmune Encephalomyelitis. Pooja Jain, Caroline Coisne, Gaby Enzmann, Robert Rottapel, and Britta Engelhardt. J. Immunol. Jun 2010; 184: 7196 - 7206. PubMed.

4. External Resources

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Anti-human CD326 (EpCAM), clone 9C4

The9C4 (monoclonal antibody recognizes human CD326 also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges, and one THYRO domain. This cell surface, glycosyl;ated 40kD protein is highly expressed in the bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. The CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas. The 9C4 antibody has been shown to be useful for flow cytometry.

Figure Legend: Human colon carcinoma cell line HT29 stained with 9C4 PerCP/Cy5.5.

Anti-β-catenin, Poly6001

β-catenin is a 94 kD nuclear and cytoplasmic protein containing Armadillo repeats. This protein is involved in cell-cell adhesion, wingless/WNT signaling pathway, and has been implicated as an oncogene. β-catenin can be modified by glycosylation, phosphorylation, and ubiquitination to target for degradation. This protein has been shown to interact with E-cadherin, α-catenin, axin, APC, GSK-3β, and Tcf/Lef. Dissociation from axin allows cytoplasmic accumulation, after nuclear translocation binds Tcf/Lef. The Poly6001 antibody has been reported to be useful for Western blotting and immunofluorescence of the mouse and human β-catenin protein.

Figure Legend: Immunofluorescent microscope analysis of Hela cells using anti β-catenin polyclonal antibody (red). Nuclei were stain with DAPI (blue).

Anti-mouse / rat CD29, clone HMß1-1

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMβ1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.

Figure Legend: C57BL/6 mouse splenocytes stained with HMβ1-1 PE

Anti-mouse CD54, clone YN1/1.7.4

CD54 is also known as ICAM-1 and Ly-47. It is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, and inhibits cell-cell adhesion, antigen presentation to T cells, and leukocyte migration to inflammatory tissues.

Figure Legend: C57BL/6 mouse splenocytes stained with YN1/1.7.4 Pacific Blue™

Anti-mouse CD84, clone mCD84.7

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production.

Figure Legend: C57BL/6 mouse splenocytes stained with mCD84.7 PE

Anti-p130 Cas, Poly6139

p130 Cas (Crk-associated substrate) is a 120-130 kD docking protein and a central coordinator for tyrosine-kinase-based signaling related to cell adhesion. p130 Cas has been implicated in induction of cell migration, growth factor stimulation, cytokine receptor engagement, cardiovascular development, and actin filament assembly. p130 Cas is localized to focal adhesions and stress fibers. The Poly6139 antibody recognizes the C-terminal region of human p130 Cas and has been shown to be useful for Western blotting.

Figure Legend: Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-p130 Cas antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Purified anti-Galectin 3, Poly6132

Galectin 3 (also known as galactose-specific lectin 3 and Mac-2 antigen) is a 32 kD member of the galaptin (S-lectin) family, BH-1, that contains carbohydrate recognition domains. Galectin 3 has been shown to modulate allergic reactions and regulate mRNA splicing activity, cell cycle control, and cell adhesion. Galectin 3 has been shown to interact with IgE, galactose, casein kinase I, laminin, mucin, β-galactoside residues of cell surface, matrix glycoproteins, and some intracellular proteins. The Poly6132 antibody recognizes human and mouse galectin 3 and has been shown to be useful for Western blotting.

Figure Legend: Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Galectin 3 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.