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Cell adhesion molecules are necessary for many different cellular functions including cell to cell signaling, cell migration, cell rolling, activation, cell killing, apoptosis, maturation, formation of tight junctions, as well as other functions.  Understanding cell adhesion is important in fully elucidating immune cell functions.  Biolegend provides an array of antibodies against adhesion molecules for the study of cell adhesion, including applications such functional assays, western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.       

Human Cell Adhesion Molecules

Mouse Cell Adhesion Molecules.

Interactive Human CD Molecule chart

Interactive Mouse CD Molecule chart

1. β1 integrins differentially control extravasation of inflammatory cell subsets into the CNS during autoimmunity. Martina Bauer, Cord Brakebusch, Caroline Coisne, Michael Sixt, Hartmut Wekerle, Britta Engelhardt, and Reinhard Fässler. PNAS. Feb 2009; 106: 1920 - 1925. PubMed

2. Ambient Air Pollution Exaggerates Adipose Inflammation and Insulin Resistance in a Mouse Model of Diet-Induced Obesity. Qinghua Sun, Peibin Yue, Jeffrey A. Deiuliis, Carey N. Lumeng, Thomas Kampfrath, Michael B. Mikolaj, Ying Cai, Michael C. Ostrowski, Bo Lu, Sampath Parthasarathy, Robert D. Brook, Susan D. Moffatt-Bruce, Lung Chi Chen, and Sanjay Rajagopalan. Circulation. Feb 2009; 119: 538 - 546. PubMed

3. Surfactant protein-A modulates cell surface expression of CR3 on alveolar macrophages and enhances CR3-mediated phagocytosis. Malgorzata Gil, Francis X. McCormack, and Ann Marie LeVine. J. Biol. Chem. Jan 2009; 10.1074/jbc.M808643200. PubMed

4. NKp80 defines and stimulates a reactive subset of CD8 T cells. Sabrina Kuttruff, Sven Koch, Alexandra Kelp, Graham Pawelec, Hans-Georg Rammensee, and Alexander Steinle. Blood. Jan 2009; 113: 358 - 369. PubMed

5. The extracellular matrix protein mindin regulates trafficking of murine eosinophils into the airspace. Zhuowei Li, Stavros Garantziotis, Wei Jia, Erin N. Potts, Sikander Lalani, Zhi Liu, You-Wen He, W. Michael Foster, and John W. Hollingsworth. J. Leukoc. Biol. Jan 2009; 85: 124 - 131. PubMed

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The 9C4 monoclonal antibody recognizes human CD326 also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges, and one THYRO domain. This cell surface, glycosyl;ated 40kD protein is highly expressed in the bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. The CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas. The 9C4 antibody has been shown to be useful for flow cytometry.


Human colon carcinoma cell line HT29 stained with 9C4 PerCP/Cy5.5.

β-catenin is a 94 kD nuclear and cytoplasmic protein containing Armadillo repeats. This protein is involved in cell-cell adhesion, wingless/WNT signaling pathway, and has been implicated as an oncogene. β-catenin can be modified by glycosylation, phosphorylation, and ubiquitination to target for degradation. This protein has been shown to interact with E-cadherin, α-catenin, axin, APC, GSK-3β, and Tcf/Lef. Dissociation from axin allows cytoplasmic accumulation, after nuclear translocation binds Tcf/Lef. The Poly6001 antibody has been reported to be useful for Western blotting and immunofluorescence of the mouse and human β-catenin protein.


Immunofluorescent microscope analysis of Hela cells using anti β-catenin polyclonal antibody (red). Nuclei were stain with DAPI (blue).

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMβ1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.


C57BL/6 mouse splenocytes stained with HMβ1-1 PE

CD54 is also known as ICAM-1 and Ly-47. It is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, and inhibits cell-cell adhesion, antigen presentation to T cells, and leukocyte migration to inflammatory tissues.


C57BL/6 mouse splenocytes stained with YN1/1.7.4 Pacific Blue™

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production.


C57BL/6 mouse splenocytes stained with mCD84.7 PE

p130 Cas (Crk-associated substrate) is a 120-130 kD docking protein and a central coordinator for tyrosine-kinase-based signaling related to cell adhesion. p130 Cas has been implicated in induction of cell migration, growth factor stimulation, cytokine receptor engagement, cardiovascular development, and actin filament assembly. p130 Cas is localized to focal adhesions and stress fibers. The Poly6139 antibody recognizes the C-terminal region of human p130 Cas and has been shown to be useful for Western blotting.


Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-p130 Cas antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Galectin 3 (also known as galactose-specific lectin 3 and Mac-2 antigen) is a 32 kD member of the galaptin (S-lectin) family, BH-1, that contains carbohydrate recognition domains. Galectin 3 has been shown to modulate allergic reactions and regulate mRNA splicing activity, cell cycle control, and cell adhesion. Galectin 3 has been shown to interact with IgE, galactose, casein kinase I, laminin, mucin, β-galactoside residues of cell surface, matrix glycoproteins, and some intracellular proteins. The Poly6132 antibody recognizes human and mouse galectin 3 and has been shown to be useful for Western blotting.


Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Galectin 3 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Specificity Clone Isotype Applications
14-3-3 γ/δ Poly6148 Rabbit IgG WB
β-catenin Poly6001 Rabbit IgG IF,WB
CLA HECA-452 Rat IgM, κ FC, IHC(Frozen/Paraffin)1,2
Dystroglycan-Phosphorylated (Tyr893) Poly6171 Rabbit IgG WB
CD147 HIM6 Mouse IgG1, κ FC, WB1, IHC(Frozen/Paraffin)1,3
CD105 MEM-226 Mouse IgG2a, κ FC1, WB (non-reducing)1, IP1
CD105 MJ7/18 Rat IgG2a, κ FC, WB, IP, IHC(Frozen)2-4
FAK Poly6038 Rabbit IgG WB1, IF1
Galectin 3 Poly6132 Rabbit IgG WB
CD42b HIP1 Mouse IgG1, κ FC, WB, IHC(Frozen)
CD41 HIP8 Mouse IgG1, κ FC, IHC(Frozen)
CD44 IM7 Rat IgG2b, κ FC, IP1,3, ELISA2, IHC(Frozen/ Paraffin)6,7
CD54 1A29 Mouse IgG1, κ FC, IHC(Frozen/ Paraffin)1,5, IP2
CD54 MEM-111 Mouse IgG2a, κ FC1, ICFC2, WB3, IHC (Frozen/ Paraffin)4,5
CD54 YN1/1.7.4 Rat IgG2b, κ FC, IHC(Frozen)3, IP4, WB(non-reducing)
CD102 3C4 (MIC2/4) Rat IgG2a, κ FC, IP1, IHC(Frozen)2
CD50 MEM-171 Mouse IgG1, κ FC, IP
ILK-1 Poly6043 Rabbit IgG WB
CD49b AK-7 Mouse IgG1, κ FC, IHC(Frozen)
CD49b/Pan-NK Cells DX5 Rat IgM, κ FC1,3,4, IHC(Paraffin)5
hma2, HMalpha2, integrina HMα2 Armenian Hamster IgG FC, IP1, IHC(Frozen)
CD49d 9C10(MFR4.B) Rat IgG2a, κ FC4, IHC(Frozen)3
CD49d 9F10 Mouse IgG1, κ FC, IHC(Frozen)
CD49d MRα4-1 Mouse IgG2a, κ FC, IP1
CD49d R1-2 Rat IgG2b, κ FC, IP1, IHC(Frozen)5
CD49e, hmalpha5-1, hma5-1 HMα5-1 Armenian Hamster IgG FC, IP1
CD49e 5H10-27(MFR5) Rat IgG2a, κ FC8, IHC(Frozen)6,7
CD49f GoH3 Rat IgG2a, κ FC1, IHC(Frozen)2,3,5, IP1,5
CD11a 2D7 Rat IgG2a, κ FC4, IHC(Frozen)1
CD11a HI111 Mouse IgG1, κ FC, IHC(Frozen), WB2
CD11a WT.1 Mouse IgG2a, κ FC, IHC1,2, IP3
CD11a M17/4 Rat IgG2a, κ FC4, IHC(Frozen)3, IP1,2
CD11b ICRF44 Mouse IgG1, κ FC, IHC(Frozen), IF5
CD11b M1/70 Rat IgG2b, κ FC6-9, IP1, IHC(Frozen)3-5
CD11b (Activated Form) CBRM1/5 Mouse IgG1, κ FC, IP
CD11b/c OX-42 Mouse IgG2a, κ FC
CD51 H9.2B8 Armenian Hamster IgG FC, IP1,3
CD51 RMV-7 Rat IgG1, κ FC, IP1
CD51/61 23C6 Mouse IgG1, κ FC, IHC(Frozen)5, IF5, IP5
CD11c 3.9 Mouse IgG1, κ FC, IHC(Frozen)4
CD11c N418 Armenian Hamster IgG FC, IP3, IHC(Frozen)3
CD29 HMß1-1 Armenian Hamster IgG FC3,5, IP1, IHC(Frozen)IHC(Frozen)4
CD29 TS2/16 Mouse IgG1, κ FC1-3,5,6, IP3, IHC(Frozen)3,5
Integrin ß1-Phosphorylated (Tyr783) Poly6006 Rabbit IgG WB
Integrin ß1-Phosphorylated (Tyr783) Poly6006 Rabbit IgG WB
Integrin ß1-Phosphorylated (Tyr795) Poly6005 Rabbit IgG WB
CD18 M18/2 Rat IgG2a, κ FC, IP1,2, WB1, IHC(Frozen/ Paraffin)7
CD18 TS1/18 Mouse IgG1, κ FC
CD18 WT.3 Mouse IgG1, κ FC, IHC(Frozen)1, IP2
CD61 2C9.G2 (HMß3-1) Armenian Hamster IgG FC, IHC(Frozen)
MAdCAM-1 MECA-367 Rat IgG2a, κ FC, IP, WB1, IHC(Frozen)1,5,6
CD56 MEM-188 Mouse IgG2a, κ FC, IP, WB, IHC (paraffin)
CD56 HCD56 Mouse IgG1, κ FC
p130 Cas Poly6139 Rabbit IgG WB
Panendothelial Cell Ag MECA-32 Rat IgG2a, κ FC, IP, WB, ICC, IHC(Frozen/Paraffin)2-4
Paxillin Poly6007 Rabbit IgG WB1, IF1
Paxillin-Phosphorylated (Tyr31) Poly6008 Rabbit IgG WB1
Paxillin-Phosphorylated (Tyr118) Poly6009 Rabbit IgG WB1
CD31 390 Rat IgG2a, κ FC, IP1, IHC(Frozen/ Paraffin)5,6
CD31 WM59 Mouse IgG1, κ FC, IHC(Frozen), IF2
CD31 MEC13.3 Rat IgG2a, κ FC, IP1, IHC(Frozen/ Paraffin)1,5,6
PNAd MECA-79 Rat IgM, κ IP3,5, WB6, IHC(Frozen/Paraffin)1,2,6
CD62L DREG-56 Mouse IgG1, κ FC, WB2,3, IHC(Frozen)
CD62L MEL-14 Rat IgG2a, κ FC7,10, IP1-3, IHC(Frozen/ Paraffin)6
CD62P AK4 Mouse IgG1, κ FC, IHC(Frozen)
CD22 HIB22 Mouse IgG1, κ FC, IHC(Frozen)
c-Src Poly6054 Rabbit IgG WB1
Src Phosphorylated (Tyr419) Poly6055 Rabbit IgG WB