|
|
|
|
T Regulatory Cells |
T regulatory cells, expressing the Foxp3 transcription factor, are essential in the maintenance of immune homeostasis and self-tolerance. This subgroup of specialized T cells limits immune activation through a variety of direct and indirect interactions, many of which remain to be defined. Fully understanding Tregs will lead us to harnessing the capacity of these cells in order to develop strategies to limit and prevent autoimmune disorders, tolerance to transplantation, and potentially boosting immune activity against cancer cells. BioLegend is proud to offer antibody products for identification and study of mouse and human Treg cells.
See all human Treg-related products.
See all mouse Treg-related products.
|
 |
1. Foxp3 Structure and Antibody Binding Sites |
F1. Foxp3 Structure and Antibody Binding Sites FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D antibody recognizes FOXP3 epitope encoded by exon 2 in humans, mouse and rat T regs. Clone 206D and 259D recognize a human FOXP3 epitope in the region of amino acids 105-235. Poly6238 recognizes both human and mouse FOXP3 and was raised against the N-terminal amino acid sequence: KPMAPSLALGPSC. |
|
|
|
|
Download a pdf of our Treg poster.
Order your complimentary poster today and start decorating your lab! |
|
|
|
3. Explore our Treg-specific cell markers |
Click on the proteins to view products.
|
|
|
|
4. View our Cell Pathways |
Immunologic Networks
Th1 Pathway (Cellular Immune Response)
Th2 Pathway (Humoral Immune Response)
Th17 Pathway
All Pathways |
|
|
|
5. View our Research Guides and Protocols. |
T Regulatory Cells Resource Guide
Intracellular Cytokine Staining Resource Guide
Interactive Human CD Molecule chart
Interactive Mouse CD Molecule chart
FOXP3 Intracellular Staining Protocol
Cell Surface Immunofluorescence Staining Protocol
Intracellular Cytokine Staining Protocol
FAQs
|
|
|
|
6. Read the latest Treg references using BioLegend products |
|
1. Defects in IL-2R Signaling Contribute to Diminished Maintenance of FOXP3 Expression in CD4+CD25+ Regulatory T-Cells of Type 1 Diabetic Subjects. S. Alice Long, KAren Cerosaletti, Paul L. Bollyky, Megan Tatum. Heather Shilling, Sheng Zheng, Zhong-Yin Zhang, Catherine Pihoker, Srinath Sandra, Carla Greenbaum and Jane H. Buckner. Diabetes. Feb 2010;59:407-415. PubMed. |
|
2. Expansion of FOXP3+ CD8 T cells with suppressive potential in colorectal mucosa following a pathogenic simian immunodeficiency virus infection correlates with diminished antiviral T cell response and viral control.Pragati Nigam, Vijayakumar Velu, Sunil Kannanganat, Lakshmi Chennareddi, Suefen Kwa, Mariam Siddiqui, and Rama Rao Amara.J. Immunol. Feb 2010; 184:1690-1701. PubMed. |
|
3. Transient role of CD4+CD25+ regulatory T cells in mycobacterial infection in mice.Yuriko Ozeki, Isamu Sugawara, Tadashi Udagawa, Toshiaki Aoki, Mayuko Osada-Oka, Yoshitaka Tateishi, Hajime Hisaeda, Yuji Nishiuchi, Nobuyuki Harada, Kazuo Kobayashi, and Sohkichi Matsumoto. Int. Immunol. Mar 2010; 22:179-189. PubMed. |
|
4. The role of the LAT-PLC-gamma1 interaction in T regulatory cell function. Mariana I. Chuck, Minghua Zhu, Shudan Shen, and Weiguo Zhang. J. Immunol. Mar 2010; 184: 2476-2486. PubMed. |
|
5. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. Ye H. Oo, Chris J. Weston, Patricia F. Lalor, Stuart M. Curbishley, David R. Withers, Gary M. Reynolds, Shishir Shetty, Jehan Harki, Jean C. Shaw, Bertus Eksteen, Stefan G. Hubscher, Lucy S. K. Walker, and David H. Adams. J. Immunol. Mar 2010; 184: 2886-2898. PubMed. |
|
|
|
|
|
View External Resources Page
View Flow Cytometry Resources Page |
|
|
|
a. Anti-human FOXP3, clone 206D |
 |
FOXP3
is a 50 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.
Figure Legend: Human peripheral blood lymphocytes surface stained with CD4 FITC and then intracellular stained with 206D PE.
|
|
|
|
|
b. Anti-human FOXP3 Flow Kit, clone 259D |
The 259D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.
BioLegend's anti-human FOXP3 Flow™ Kits
are designed and formulated specifically for immunofluorescence staining and flow cytometric analysis of human FOXP3+ Treg cells in a mixed lymphocyte population. This kit is composed of Alexa Fluor® 488 conjugated anti-human FOXP3 antibody and Alexa Fluor® 488 conjugated matching isotype control and the critical buffers. It is easy to use for identification of FOXP3+ Treg cells.
Figure Legend: Human peripheral blood lymphocytes surface stained with CD4 APC and then intracellular stained with 259D Alexa Fluor® 488 by using Alexa Fluor® 488 anti-human FOXP3 flow kit. Quadrant markers were set based on the staining with Alexa Fluor® 488 mIgG1, κ isotype control.
|  |
|
|
|
|
c. Anti-mouse/human Helios, clone 22F6 |
 |
Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. Importantly, it is found at high levels in FOXP3+ regulatory T cells from the Thymus but not peripherally induced Tregs. Helios also plays an important role in T cell development and homeostasis.
Figure Legend: C57BL/6 splenocytes intracellular stained with 22F6 PE and CD4 (GK1.5) APC using FOXP3 Fix/Perm Buffer Set (cat# 421403).
|
|
|
|
|
d. One Step Staining Human Treg Flow™ Kit |
BioLegend’s One Step Staining Human Treg Flow™ Kits
are designed and formulated specifically for immunofluorescence staining and flow cytometric analysis of human Treg cells in a mixed lymphocyte population. This kit is composed of fluorochrome conjugated anti-human CD4, CD25 and FOXP3 antibodies and the critical buffers. It is a convenient one-step staining kit for labeling both surface and intracellular antigens of Treg cells.
Figure Legend: Human PBMCs were one-step stained intracellularly with anti-CD25-PE and 206D Alexa Fluor® 488.
|  |
|
|
|
|
e. Anti-mouse GITR, clone YGITR 765 |
 |
GITR, Glucocorticoid-induced TNFR-related gene, is expressed at high levels on CD4+CD25+ T regulatory (Treg) cells. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4+CD25+ Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses. The YGITR 765 antibody is recommended for flow cytometry applications.
Figure Legend: C57BL/6 mouse splenocytes stained YGITR 765 FITC and CD25 APC.
|
|
|
|
|
f. Anti-human CD127, clone HCD127 |
CD127 is also known as IL-7 receptor α chain or IL-7Rα. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Importantly, CD127 expression is down-modulated on Treg cells. Thus, it can be used as a marker for differentiation of Treg and conventional T cells without the need for fixation and permeabilization of cells, allowing the researcher to sort for enriched populations of Tregs. The HCD127 antibody is recommended for flow cytometry applications.
Figure Legend: Human peripheral blood lymphocytes stained with CD3 PE and HCD127 Alexa Fluor® 647.
|  |
|
|
|
|
|
|
|
|
|
| |
| |
| |
| |
| |
    |
| Copyright © 2010 BioLegend, Inc. All rights reserved. |
|
|
