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Human TruStain FcX™ (Fc Receptor Blocking Solution)
Human TruStain FcX™ (Fc Receptor Blocking Solution)
422301 50 tests $85.00     
422302 200 tests $195.00     
Product Details
Storage & Handling: Store at 4°C.
Application:

FC - Quality tested
ICFC, ICC - Reported in the literature
Recommended Usage:

It is recommended to add 5 µl of Human TruStain FcX™ per 100 µl of cell suspension, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.
COA:
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Application
Notes:

This buffer contains specialized human IgG. It is not recommended to be used for staining human IgG. Handle as biohazard agent.
Application
References:

  Publication Library
Human TruStain FcX™ (Fc Receptor Blocking Solution) Image 1
Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG1 PE mAb (above). Dotted histogram represents unstained cells.

Human TruStain FcX™ (Fc Receptor Blocking Solution) Image 2
Human TruStain FcX™ treated (filled histograms) or non-treated (open histograms) THP-1 cells stained with anti-human HLA-DR PE (red) or an isotype control (IgG2a PE, blue) mAbs. Note: non-treated cells show false-negative HLA-DR staining due to high binding of mouse IgG1 isotype.


Description:

Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells. The cells with FcR expression sometimes give false positive or false negative results of immunofluorescent staining due to the FcRs-mediated Ig Fc binding. Human TruStain FcX™ is specially formulated for blocking the FcR-involved unwanted staining without interfering with antibody-mediated specific staining of human cells.
Other Names: Fc Blocker, FcγR blocker, Fc inhibitor
GeneID: NA
Latest Publications: View the latest articles on HighwirePress.com
Keywords: Human TruStain FcX™ (Fc Receptor Blocking Solution), Solution, Fc Blocker, FcγR blocker, Fc inhibitor , Flow Cytometry, Immunocytochemistry, Intracellular Staining for Flow Cytometry, Biofunctional Assay, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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