Antibodies
Account Login LEGENDClub
Search 
 
Products Site Search
Advanced Search Shopping Cart
Home Company Products Custom Assays Pathways Support Contact

Human FoxP3 Transfected Cell Lysate
Material Safety Data Sheet (MSDS) Human FoxP3 Transfected Cell Lysate     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Human FoxP3 Transfected Cell Lysate
629901 100 µl $75.00     
Clone: lysate6299
Formulation: The cell lysate is provided in 100 µl 1X SDS sample buffer (62.5 mM Tris-HCl, pH6.8; 2% SDS (w/v), 50 mM DTT, 10% glycerol, 0.01% Bromophenol Blue (w/v))
Storage & Handling: Upon receipt, store at -20°C in a non-defrosting freezer. Avoid repeating freeze thaw cycles
Application: Western blotting standard
Recommended Usage: Aliquot upon receipt (20 µl individual sample size) and store at -20°C (stable for 1 year when stored appropriately). Minimize repeated freeze thaw cycles. Boil individual aliquot for 5 minutes before loading on gel. Load one 20 µl aliquot per mini-gel or standard gel lane.


Description: FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The human FoxP3 transfected cell lysate can be used as a positive control for Western blotting with the monoclonal anti-human FoxP3 antibodies clone 150D (Cat. No. 320001), clone 206D (Cat. No. 320101) and clone 259D (Cat. No. 320201) as well as the polyclonal anti-human FoxP3 antibody (Cat. No. 623801).
Purified anti-FOXP3Poly6238IHC,WB
Purified anti-mouse/rat/human FOXP3150DICFC,IHC,WB
Purified anti-human FOXP3206DICFC,IHC,WB
Purified anti-human FOXP3259DICFC,IHC,WB