Each lot of this antibody is quality control tested by ELISA analysis. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

This HRP conjugated polyclonal goat anti-mouse IgG antibody is useful for ELISA and Western blotting applications.

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may cross-react with other subclasses of mouse immunoglobulins.