Aqueous buffered solution containing BSA (origin USA) and 50% glycerol.
Preparation:
The polyclonal antibody was purified from goat antiserum by mouse immunoglobulin affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at -20°C. Avoid exposure to sodium azide.
Application:
ELISA - Quality Tested WB1*, IHC * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by ELISA analysis. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
This HRP conjugated polyclonal goat anti-mouse IgG antibody is useful for ELISA and Western blotting applications.
Application References:
1. Lawson, B. R., et al., 2007. J. Immunol. 178:5366.
Description:
This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may cross-react with other subclasses of mouse immunoglobulins.
