Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

This HRP conjugated polyclonal goat anti-rat IgG antibody is useful for ELISA, IHC, and Western blotting applications.