Aqueous buffered solution containing BSA (origin USA) and 50% glycerol.
Preparation:
The polyclonal antibody was purified from donkey antiserum by rabbit immunoglobulin affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at -20°C. Avoid exposure to sodium azide.
Application:
WB - Quality Tested ELISA*, IHC * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
This HRP conjugated polyclonal donkey anti-rabbit IgG antibody is useful for ELISA, IHC and Western blotting applications.
Application References:
1. Lawson, B. R., et al., 2007. J. Immunol. 178:5366.
Description:
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, mouse, human, bovine, horse, goat, chicken, guinea pig, Syrian hamster, and sheep immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
