Anti-α-Synuclein Antibody (Previously Covance catalog# MMS-530R)

Pricing & Availability
Clone
Syn 204 (See other available formats)
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease (autosomal dominant, Lewy body) 4
Previously
Covance Catalog# MMS-530R
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A_Syn204_Ascites_a-Synuclein_IHC_110918
IHC staining of anti-α-Synuclein antibody (clone Syn 204) on formalin-fixed paraffin-embedded human Parkinson's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_Syn204_Ascites_a-Synuclein_IHC_110918
    IHC staining of anti-α-Synuclein antibody (clone Syn 204) on formalin-fixed paraffin-embedded human Parkinson's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:5000 dilution of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_Syn204_Ascites_a-Synuclein_WB_11092018
    Western blot of anti-α-Synuclein antibody (clone Syn 204). Lane 1: Molecular weight marker; Lane 2: 30 µg of human Parkinson's disease brain lysate; Lane 3: 20 µg of normal human brain lysate. The blot was incubated with a 1:2000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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838202 25 µl 100€
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838201 200 µl 256€
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Description

Alpha-synuclein is expressed principally in the central nervous system (brain) but is also expressed in low concentrations in a variety of tissues except liver. It is predominantly expressed in the neocortex, hippocampus, substantia nigra, thalamus, and cerebellum of the CNS. It is primarily a neuronal protein, but can also be found in the neuroglial cells. It is concentrated in presynaptic nerve terminals of neurons, as well as having reported nuclear and mitochondrial localization. Alpha-synuclein interacts with plasma membrane phospholipids. Alpha-synuclein in solution is considered to be an intrinsically disordered protein and thus lacks a stable secondary or tertiary structure. However, recent data suggests the presence of partial alpha helical as well as beta sheet structures as well as mostly structured tetrameric states in solution, the equilibrium of which may be altered by binding partners. The human alpha-synuclein protein is made of 140 amino acids, encoded by the SNCA gene. The primary structure is divided in three distinct domains: (1-60) - An amphipathic N-terminal region dominated by four 11-residue repeats including the consensus sequence KTKEGV. This sequence has a structural alpha helix propensity similar to apolipoproteins-binding domains. (61-95)- a central hydrophobic region which includes the non-amyloid-β component (NAC) region, involved in protein aggregation. (96-140)- a highly acidic and proline-rich region. At least three isoforms of synuclein are produced through alternative splicing. The most common form of the protein, is the full 140 amino acid-long transcript. Other isoforms are alpha-synuclein-126, lacking residues 41-54; and alpha-synuclein-112, which lacks residues 103-130. a-synuclein may be involved in the regulation of dopamine release and transport and also may function to induce fibrillization of microtubule-associated protein tau. alpha-synuclein functions as a molecular chaperone in the formation of SNARE complexes. In particular, it can bind to phospholipids of the plasma membrane and to synaptobrevin-2 via its C-terminus domain to influence synaptic activity. Alpha-synuclein is essential for normal development of the cognitive functions and that it significantly interacts with tubulin. It also reduces neuronal responsiveness to various apoptotic stimuli, leading to decreased caspase-3 activation. Alpha Synuclein fibrils are major substituent of the intracellular Lewy bodies seen in Parkinson’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Syn 204 was raised using recombinant alpha synuclein and recognizes residues 1-130 of alpha synuclein.
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
Application

IHC-P - Quality tested
WB - Validated
ICC - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested. For Western blotting, the suggested use of this reagent is 1:1000 - 1:2000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC-P), and immunocytochemistry (ICC).

Application References
  1. Yazawa I, et al. 2005. Neuron. 45:847
  2. Giasson BI, et al. 2000. J Neurosci Res. 59(4):528
  3. Follenzi A, et al. 2008. J. Clin. Invest. 2:777. (WB)
  4. Galvin JE, et al. 2000. Am. J. Pathol. 2:361. (IHC-P)
  5. Luk KC, et al. 2014. Nat. Protoc. 9:2135. (ICC)
  6. Lippa CF, et al. 2001. Arch. Neurol. 11:1817. (IHC-P)
  7. Liu Y, et al. 2014. F1000Res. 3:68. (WB)
RRID
AB_2734614 (BioLegend Cat. No. 838202)
AB_2565390 (BioLegend Cat. No. 838201)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 11/09/2018

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