MojoSort™ is BioLegend's magnetic cell separation system for the isolation and purification of cells from heterogeneous populations. MojoSort™ offers outstanding performance at an excellent price. Add some mojo to your experiments today! Learn more about BioLegend’s cell separation system below.

About MojoSort™

What's in the name? From the Merriam-Webster Dictionary, mojo is a power that may seem magical and allows someone to be very effective or successful. And that’s what we believe: that by using MojoSort™ you will get great results in your cell separation experiment, and at the same time, save on your budget.

MojoSort™ Nanobeads are magnetic particles directly conjugated to antibodies (positive selection) or Streptavidin. MojoSort™ Isolation Kits typically contain a biotin-antibody cocktail and Streptavidin Nanobeads, intended to isolate an untouched cell population. To use these products, you also need a separation buffer and a magnetic separation system. We recommend our MojoSort™ Buffer (Cat. No. 480017) and MojoSort™ Magnet (Cat. No. 480019).

mojo magnet image

Free MojoSort™ Magnet

If you are interested in trying out our kits and need a loaner magnet, please contact your local BioLegend representative or our inside  sales team to receive a MojoSort™ Magnet (5 ml size, catalogue item 480019). New customers that decide to keep on using the MojoSort™ reagents can keep the magnet. Existing MojoSort™ customers can receive a free magnet with the purchase of three large size MojoSort kits. To redeem this offer, customers must contact their local BioLegend representative or our inside sales team to get a quote for the three kits and the free magnet. Offer valid for direct end users in USA, Canada, UK, Ireland, Germany, Austria, Switzerland, France, Belgium, the Netherlands, and Luxembourg.  Restrictions may apply. Other countries, please contact your distributor if interested. 

The isolation of a specific cell population from a complex sample has become a common lab procedure. The availability of highly specific antibodies and the demand for targeted applications keeps on pushing the limits of the cell separation field. Thus far, three major techniques have been used for cell separation: centrifugation-based methods, fluorescence-activated cell sorting (FACS), and magnetic cell sorting. FACS and magnetic cell sorting offer the advantage of a more specific isolation, as they use antibodies to select the target population. However, they differ fundamentally in the way they work. So how does magnetic cell separation work?

 

Basically, specific antibodies that are conjugated to magnetic particles will bind to the cells expressing the target antigen. Applying a magnetic field will attract the cells towards the magnet, separating them from the rest of the cells. Alternatively, a 2-step separation process can be done. For example biotin-antibody conjugates followed by streptavidin-conjugated beads, or a similar approach, can also be used.

protocol video image

View MojoSort™ General Protocol Video…

 

Frequently Asked Questions


Please review the following common questions to learn more about the MojoSort™ Magnetic Separation System, its properties, usage and applications. Contact our Technical Service Group if you need more information.

 

Email: Click Here
Toll-Free (US & Canada): 1-877-273-31031-877-273-3103 FREE
Phone (International): 1-858-768-58011-858-768-5801
 

 

What is the shelf-life of your magnetic particles?


1 to 2 years depending on product type.

 

What is the coating on your magnetic particles?


Hydrophilic polymers.

 

Can your magnetic particles withstand freeze/thaw cycles?


Not recommended, however lyophilized particles can be made available as a custom product.

 

What is the size of your magnetic particles?


The average diameter is approximately 130 nm.

 

What is the guarantee time of your magnetic particles?


A minimum of 6 months from the date of purchase.

 

Do your magnetic particles get internalized by the cells?


We have not determined this yet.

 

Are downstream applications affected by the magnetic beads bound to the cells?


We have tested mouse CD4 positive cells that have been isolated from the spleen and lymph nodes with directly conjugated Nanobeads, as well as CX3CR1 expressing cells from mouse bone marrow. The cells appear unaffected as assessed by migration, differentiation and stimulation assays.

 

Will your magnetic particles separate well in other company’s magnetic separation systems?


It is possible that other magnetic separation systems can be used. To learn more about this please contact our Technical Service Group.

 

Do you provide a custom conjugation service to your magnetic particles?


Yes, we do. Please contact our Custom Solutions Team at cst@biolegend.com.

 

Is there a way to detach your magnetic particles from the cell surface?


No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

What is the storage buffer of your particles?


The particles are stored in a neutral pH solution containing BSA and sodium azide.

 

Can your magnetic particles be sterile filtered?


Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

 

Can I stain positively sorted cells by flow cytometry?


Yes, this is possible, but if you are staining for the same marker as used for sorting, we would recommend using a different clone with non-overlapping epitopes.

 

Are there any clone restrictions when checking the purity of isolated cells?


There are no restrictions for negatively selected cells, as they are untouched by antibodies. For positive selection, there may be clones that are less efficient than others due to possible epitope competition. Please refer to the Technical Data Sheet or contact the Technical Service Group for advice.

 

Are your magnetic particles suitable for use with whole blood?


We have not tested this application yet.

 

What is the composition of your MojoSort™ Buffer?


The diluted, 1X MojoSort™ buffer contains 1X phosphate buffer saline (PBS) supplemented with 2 mM EDTA and 0.5% BSA. The 5X solution is sterile. To preserve sterility dilute with sterile water under sterile conditions.

 

Do you offer your base magnetic particles (i.e. without any streptavidin or antibody conjugation)?


No, not currently.

 

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?


MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance.  We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Applications

Separation of cells can occur via “Positive Selection” or “Negative Selection” (also known as “Negative Depletion”). This depends on whether the bead-bound antibodies directly target your cells of interest (positive selection) or target unwanted cells (negative selection). Either way, both separated fractions of cells can be used for downstream applications.

Positive Selection

Positive Selection

Negative Selection

Negative Selection

Labeled cells.
Keep for downstream applications.

Unlabeled cells.
Discard or keep as controls if needed.

Unlabeled, untouched isolated cells.
Keep for downstream applications.

Labeled cells.
Discard (or keep as controls if needed).


particle properties image

MojoSort™ Isolation Kits Provide Excellent Purity

MojoSort™ Mouse CD4 T Cell Isolation Kit

 

 

A single cell suspension from pooled C57BL/6 mouse spleen and lymph nodes was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with PE anti-mouse CD4 (clone RM4-4), APC anti-mouse CD3ε (145-2C11), and 7-AAD. Dead cells were excluded from the analysis.

MojoSort™ Human CD4 T Cell Isolation Kit

 

 

A single cell suspension from pooled C57BL/6 mouse spleen and lymph nodes was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with PE anti-mouse CD4 (clone RM4-4), APC anti-mouse CD3ε (145-2C11), and 7-AAD. Dead cells were excluded from the analysis.


In Vivo Data

CX3CR1+ Cells Migrate Back to the Bone Marrow After Adoptive Transfer

cx3cr1 image

CX3CR1+ cells were isolated from C57BL/6 mice bone marrow using a two step separation protocol. Total bone marrow cells were incubated with directly conjugated Ly-6G Nanobeads to deplete granulocytes. After depletion, the resulting fraction was incubated with biotin anti-mouse CX3CR1 antibody followed by MojoSort™ Streptavidin Nanobeads, resulting in a population containing about 70% of CX3CR1-expressing cells. The cells were then labeled with CFSE and injected back into recipient C57BL/6 mice. Three days later, CFSE+ cells are found in the bone marrow of the recipient mice. Control mouse represents an animal injected with PBS.

Protocols Cat # Kit
Nanobeads
MojoSort™ Nanobeads Protocol - 1 480001, 480002 MojoSort™ Mouse CD19 Nanobeads
  480013, 480014 MojoSort™ Human CD4 Nanobeads
MojoSort™ Nanobeads Protocol - 2 480107, 480108 MojoSort™ Human CD8 Nanobeads
MojoSort™ Streptavidin Nanobeads Protocol - Negative Selection 480015, 480016 MojoSort™ Streptavidin Nanobeads
MojoSort™ Streptavidin Nanobeads Protocol - Positive Selection 480015, 480016 MojoSort™ Streptavidin Nanobeads
MojoSort™ Mouse CD45 Nanobeads Protocol - Selection 480027, 480028 MojoSort™ Mouse CD45 Nanobeads
MojoSort™ Mouse CD45 Nanobeads Protocol - Depletion 480027, 480028 MojoSort™ Mouse CD45 Nanobeads
MojoSort™ Human CD45 Nanobeads Protocol 1, (<50% CD45+) 480029, 480030 MojoSort™ Human CD45 Nanobeads
MojoSort™ Human CD45 Nanobeads Protocol 2, (>50% CD45+) 480029, 480030 MojoSort™ Human CD45 Nanobeads
MojoSort™ Mouse CD4 Nanobeads Protocol 480069, 480070 MojoSort™ Mouse CD4 Nanobeads
MojoSort™ Mouse anti-APC Nanobeads Protocol 480071, 480072 MojoSort™ Mouse anti-APC Nanobeads
MojoSort™ Mouse CD8a Nanobeads Protocol 480075, 480076 MojoSort™ Mouse CD8a Nanobeads
MojoSort™ Mouse CD11c Nanobeads Protocol 480077, 480078 MojoSort™ Mouse CD11c Nanobeads
MojoSort™ Mouse anti-PE Nanobeads Protocol 480079, 480080 MojoSort™ Mouse anti-PE Nanobeads
MojoSort™ Human CD41 Nanobeads Protocol 480083, 480084 MojoSort™ Human CD41 Nanobeads
MojoSort™ Human anti-APC Nanobeads Protocol 480089, 480090 MojoSort™ Human anti-APC Nanobeads
MojoSort™ Human anti-PE Nanobeads Protocol 480091, 480092 MojoSort™ Human anti-PE Nanobeads
MojoSort™ Human CD14 Nanobeads Protocol 480093, 480094 MojoSort™ Human CD14 Nanobeads
Isolation Kits
MojoSort™ Isolation Kits Protocol - 1 480023, 480024, 480031 MojoSort™ Mouse CD3 T Cell Isolation Kit
  480005, 480006, 480033 MojoSort™ Mouse CD4 T Cell Isolation Kit
  480007, 480008, 480035 MojoSort™ Mouse CD8 T Cell Isolation Kit
  480009, 480010 MojoSort™ Human CD4 T Cell Isolation Kit
  480021, 480022 MojoSort™ Human CD3 T Cell Isolation Kit
  480039, 480040 MojoSort™ Mouse CD4 Naive T Cell Isolation Kit
  480041, 480042 MojoSort™ Human CD4 Naive T Cell Isolation Kit
  480043, 480044 MojoSort™ Mouse CD8 Naive T Cell Isolation Kit
  480045, 480046 MojoSort™ Human CD8 Naive T Cell Isolation Kit
  480051, 480052 MojoSort™ Mouse Pan B Cell Isolation Kit
  480061, 480062 MojoSort™ Human B Cell (CD43-) Isolation Kit
  480063, 480064 MojoSort™ Human CD4 Memory T Cell Isolation Kit
  480067, 480068 MojoSort™ Human Naïve B Cell Isolation Kit
  480081, 480082 MojoSort™ Human Pan B Cell Isolation Kit
  480087, 480088 MojoSort™ Mouse Pan B Cell Isolation Kit II
MojoSort™ Isolation Kits Protocol - 3 480003, 480004 MojoSort™ Mouse Hematopoietic Progenitor Cell Isolation Kit
  480011, 480012 MojoSort™ Human CD8 T Cell Isolation Kit
  480065, 480066 MojoSort™ Human CD8 Memory T Cell Isolation Kit
MojoSort™ Isolation Kits Protocol - 4 480095, 480096 MojoSort™ Human Hematopoietic Progenitor Cell Isolation Kit
  480097, 480098 MojoSort™ Mouse Pan Dendritic Cell Isolation Kit
MojoSort™ Human CD14+ Monocytes Isolation Kit Protocol 480047, 480048 MojoSort™ Human CD14+ Monocytes Isolation Kit
MojoSort™ Mouse NK Cell Isolation Kit Protocol 480049, 480050 MojoSort™ Mouse NK Cell Isolation Kit
MojoSort™ Human NK Cell Isolation Kit Protocol 480053, 480054 MojoSort™ Human NK Cell Isolation Kit
MojoSort™ Mouse Neutrophil Isolation Kit Protocol 480057, 480058 MojoSort™ Mouse Neutrophil Isolation Kit
MojoSort™ Human Pan Monocyte Isolation Kit Protocol 480059, 480060 MojoSort™ Human Pan Monocyte Isolation Kit
Isolation Kits
MojoSort™ Isolation Kits Column Protocol - 1 480005, 480006, 480033 MojoSort™ Mouse CD4 T Cell Isolation Kit
  480007, 480008, 480035 MojoSort™ Mouse CD8 T Cell Isolation Kit
  480009, 480010 MojoSort™ Human CD4 T Cell Isolation Kit
  480021, 480022 MojoSort™ Human CD3 T Cell Isolation Kit
  480023, 480024, 480031 MojoSort™ Mouse CD3 T Cell Isolation Kit
  480039, 480040 MojoSort™ Mouse CD4 Naive T Cell Isolation Kit
  480041, 480042 MojoSort™ Human CD4 Naive T Cell Isolation Kit
  480043, 480044 MojoSort™ Mouse CD8 Naive T Cell Isolation Kit
  480045, 480046 MojoSort™ Human CD8 Naive T Cell Isolation Kit
  480051, 480052 MojoSort™ Mouse Pan B Cell Isolation Kit
  480061, 480062 MojoSort™ Human B Cell (CD43-) Isolation Kit
  480063, 480064 MojoSort™ Human CD4 Memory T Cell Isolation Kit
  480067, 480068 MojoSort™ Human Naïve B Cell Isolation Kit
  480081, 480082 MojoSort™ Human Pan B Cell Isolation Kit
  480087, 480088 MojoSort™ Mouse Pan B Cell Isolation Kit II
MojoSort™ Isolation Kits Column Protocol - 3 480003, 480004 MojoSort™ Mouse Hematopoietic Progenitor Cell Isolation Kit
  480011, 480012 MojoSort™ Human CD8 T Cell Isolation Kit
  480065, 480066 MojoSort™ Human CD8 Memory T Cell Isolation Kit
MojoSort™ Isolation Kits Column Protocol - 4 480095, 480096 MojoSort™ Human Hematopoietic Progenitor Cell Isolation Kit
  480097, 480098 MojoSort™ Mouse Pan Dendritic Cell Isolation Kit
MojoSort™ Human CD14+ Monocyte Isolation Kit Column Protocol 480047, 480048 MojoSort™ Human CD14+ Monocytes Isolation Kit
MojoSort™ Mouse NK Cell Isolation Kit Column Protocol 480049, 480050 MojoSort™ Mouse NK Cell Isolation Kit
MojoSort™ Human NK Cell Isolation Kit Column Protocol 480053, 480054 MojoSort™ Human NK Cell Isolation Kit
MojoSort™ Mouse Neutrophil Isolation Kit Column Protocol 480057, 480058 MojoSort™ Mouse Neutrophil Isolation Kit
MojoSort™ Human Pan Monocyte Isolation Kit Column Protocol 480059, 480060 MojoSort™ Human Pan Monocyte Isolation Kit

Usage With Columns

Due to the properties of our magnetic nanobeads, it is possible to use them in commercially available separation columns. We have validated their use with such devices through internal research, and confirmed at academic labs. It is also possible to use them in column-based automatic separation machines. Thanks to their strong magnetic properties, the number of beads needed to separate the cells may be far lower than particles from other commercial suppliers. Thus, to use MojoSort™ Nanobeads in commercially available columns, a dilution is recommended. Click in the corresponding buttons for Positive or Negative Selection Protocol and follow the instructions as a guideline for optimal results. If you need further assistance please contact BioLegend Technical Support.

Electron Microscopy With Separation Columns

Positively Selected Cells

 

 

MojoSort™ Nanobead-isolated CD19+ cells using columns do not display more bound beads on the cell surface (A) as compared to cells isolated with a compatible commercial product using the same columns (B). Red arrows indicate where the particles are located. Numbers indicate either 2 or 3 magnetic particles adjacent to each other. Pictures were taken at the same magnification, scale shown in B. Images are representative of 41 different cells each.


Negatively Selected Cells

 

 

CD4+ T cells Isolated with MojoSort™ CD4 T Cell Isolation Kit using columns do not display particles in the cell surface. Image is representative of 36 different cells.

 

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