Purified anti-α-Synuclein, aggregated Antibody

Pricing & Availability
Clone
Syn-O2 (See other available formats)
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype
Mouse IgG1, κ
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Product Citations
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Syn-O2_PURE_alpha-Synuclein_Antibody_1_IHCP_111016
IHC staining of α-Synuclein deposits with anti-α-Synuclein, aggregated antibody (clone Syn-O2) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using citrate and 0.25% Triton X-100, the tissue was incubated with the primary antibody at 1 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • Syn-O2_PURE_alpha-Synuclein_Antibody_1_IHCP_111016
    IHC staining of α-Synuclein deposits with anti-α-Synuclein, aggregated antibody (clone Syn-O2) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using citrate and 0.25% Triton X-100, the tissue was incubated with the primary antibody at 1 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • Syn-O2_PURE_alpha-Synuclein_Antibody_2_DotBlot_111016
    Dot Blots of anti-α-synuclein antibodies (clones Syn-O2 and 4B12/Synuclein) and isotype-matched IgG1 control, demonstrating their binding to α-synuclein conformers (monomeric and fibrillar). Note that these antibodies do not cross-react with amyloid peptide (Aβ1-42). Blots were incubated at room temperature for two hours with 0.5 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence used for detection.
See high resolution IHC-P data...
Cat # Size Price Quantity Avail. Save
847601 25 µg 60€
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847602 100 µg 164€
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Description

α-synuclein is expressed principally in the nervous system, but it is also produced in the other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in the presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in the nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Aggregated α-synuclein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Dot Blot - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Dot Blot, a concentration range of 0.5 - 3.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-react with β- or γ-synucleins using Dot Blot as detection method.

Application References

(PubMed link indicates BioLegend citation)
  1. Majbour NK, et al. 2016. Mol. Neurodegener. 11:7. (IHC, ELISA Capture, Dot Blot)
  2. Vaikath NN, et al. 2015. Neurobiol Dis. 79:81. (IHC, ELISA Capture, Dot Blot)
RRID
AB_2632700 (BioLegend Cat. No. 847601)
AB_2632701 (BioLegend Cat. No. 847602)

Antigen Details

Structure
α-synuclein's canonical isoform consists of 140 amino acids, which consists of four 11-residue repeats containing the consensus sequence KTKEGV. While, α-synuclei has an apparent molecular mass of 14 kD.
Distribution

Cellular distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extraceullar.
Tissue distribution: primarily in the nervous system, but is lower expressed in other tissues.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Antigen References

1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66.
2. Jucker M, Walker LC. 2013. Nature. 501(7465):45.
3. Bartels T, et al. 2011. Nature. 477(7362):107.
4. Devine MJ, et al. 2011. Mov. Disord. 26:2160.

Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein, aggregated on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/07/2016

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