Biotin anti-Syntaxin Antibody

Pricing & Availability
Clone
SP8 (See other available formats)
Other Names
Syntaxin-1A, neuron-specific antigen HPC-1
Isotype
Mouse IgG1
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Product Citations
publications
SP8_Biotin_Syntaxin_Antibody_1_012119_updated
Western blot of Biotin anti-Syntaxin antibody (clone SP8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin. Enhanced chemiluminescence was used as the detection system.
  • SP8_Biotin_Syntaxin_Antibody_1_012119_updated
    Western blot of Biotin anti-Syntaxin antibody (clone SP8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin. Enhanced chemiluminescence was used as the detection system.
  • SP8_Biotin_Syntaxin_Antibody_2_011819.png
    Immunofluorescence staining of Biotin anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP8_Biotin_Syntaxin_Antibody_3_011819.png
    Immunofluorescence staining of Biotin anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
  • SP8_Biotin_Syntaxin_Antibody_4_011819.png
    Immunofluorescence staining of Biotin anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded rat brain hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 2.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm.
  • SP8_Biotin_Syntaxin_Antibody_5_011819.png
    Immunofluorescence staining of Biotin anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded rat brain hippocampus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 2.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
  • SP8_Biotin_Syntaxin_Antibody_6_011819.png
    Immunofluorescence staining of Biotin anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded rat brain cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 2.5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
827005 25 µg 92€
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827006 100 µg 204€
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Description

Syntaxin, also known as syntaxin-1A and neuron-specific antigen HPC-1, is part of the Q-SNARE protein family that participates in exocytosis. Syntaxins have a SNARE domain, an N-terminal regulatory domain, and a single C-terminal transmembrane domain. Syntaxin drives the spontaneous calcium-independent fusion of synaptic vesicles that contain v-SNARE proteins, and may form part of the fusion pore of exocytosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a crude synaptic preparation from a post-mortem human brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemistry, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Honer WG, et al. 1997. Neuroscience 78(1):99-110.
  2. Honer WG, et al. 1993. Brain Res. 609(1-2):9-20.

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6804 View all products for this Gene ID
UniProt
View information about Syntaxin on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 01/18/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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