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FOXP3 Fix/Perm Buffer Set
FOXP3 Fix/Perm Buffer Set
421403
100 tests
$120.00
Product Details
Storage & Handling:
The FOXP3 fix/perm buffer set solution should be stored at 4°C. Do not freeze.
The FOXP3 Fix/Perm buffer(4X) must be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer(4X) to three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Perm buffer(10X) to nine (9) parts PBS. NOTE: The FOXP3 Perm buffer (10X) may have crystalization or precipitation oberserved when it is stored at 2-8°C, however, it's normal and does not affect the buffer performance. If there is a heavy precipitation observed after diluted to 1X working solution, it can be filtered to clarify the solution.
COA:
Application Notes:
FOXP3 Intracellular Staining Procedures: 1. Perform cell surface staining as described in BioLegend's Cell Surface Immunofluorescence Staining Protocol. 2. Add 1 ml of 1X BioLegend's FOXP3 Fix/Perm solution to each tube, vortex and incubate at room temperature in the dark for 20 minutes, then spin down the cells and remove the supernatant. 3. Wash once with cell staining buffer (Cat. No. 420201) by spin at 250Xg for 5 minutes and remove the supernatant. 4. Wash once with 1ml 1X BioLegend's FOXP3 Perm buffer. 5. Re-suspend cells in 1ml 1X BioLegend's FOXP3 Perm buffer, incubate at room temperature in the dark for 15 minutes, spin down cells and discard the supernatant, then resuspend the pellet in 100 ul of 1X BioLegend's FOXP3 Perm buffer. 6. Add appropriate amount of flurochrome conjugated anti-FOXP3 antibody and incubate at room temperature in the dark for 30 minutes. 7. Wash twice with cell staining buffer, and resuspend in 0.5ml cell staining buffer then analyze with flow cytometer with appropriate instrument setting.
Application References:
1. Yang ZZ, et al. 2006. Blood 107:3639.PubMed 2. Groh V, et al. 2006. Nat. Immunol. 7:755.PubMed 3. Bamias G, et al. 2007. J. Immunol. 178:1809.PubMed 4. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249.PubMed 5. Müller M, et al. 2007. J. Immunol. 179:2774. 6. Dang Y,et al.2007.Clin Cancer Res.13:1883.PubMed 7. Basu S, et al. 2008. J. Immunol. 180:5974. PubMed 8. Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed 9. Beavis, PA., et al. 2011. PNAS. 108:16717. PubMed 10. Troutman TD, et al. 2012. PNAS. 109:273. PubMed.
Description:
BioLegend's FOXP3 Fix/Perm Buffer Set has been specially formulated for intracellular staining FOXP3 with minimum effect on the surface fluorochrome staining. It contains one 30 ml 4X concentrated FOXP3 Fix/Perm buffer (Cat. No. 421401) and one 25 ml 10X concentrated FOXP3 Perm buffer (Cat. No. 421402). The FOXP3 Fix/Perm buffer(4X) must be freshly diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer(4X) to three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Perm buffer(10X) to nine (9) parts PBS. Caution: The FOXP3 Fix/Perm buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contact.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
