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FluoroFix™ Buffer
FluoroFix™ Buffer
422101 200 tests €104     
Product Details
Storage & Handling: The buffer solution should be stored at Room Temperature (RT).
Application: FC, ICFC - Quality tested
ICC - Reported in the literature
Recommended Usage: After final wash in the staining procedures (please refer to Cell Surface Immunofluorescence Staining Protocol), discard supernatant and resuspend the cell pellet in 0.5 ml/tube FluoroFix™ Buffer and analyzed with flow cytometry. If the samples can not be analyzed within 4 hours, resuspend the cell pellet in 0.5 ml/tube FluoroFix™ Buffer and incubate in the dark for 30 minutes at room temperature. Wash 1X with Biolegend Cell Staining Buffer (cat# 420201) and resuspend in 0.5ml/tube Cell Staining Buffer, cover the tubes.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
COA:
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Description:

FluoroFix™ Buffer is a ready-to-use buffer, specially formulated for fixation and stabilization of immunofluoroscence stained cells. It can be used as the final resuspension the cell pellet in immunofluoroscence staining procedures. FluoroFix™ Buffer is provided as 100 mL.This quantity is sufficient for 200 tests.
Other Names: Fixative, Paraformaldehyde
Antigen
References: 		1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Kang YJ, et al. 2007. Nature Immunol. 8:601.
GeneID: NA
Latest Publications: View the latest fluorofix buffer articles on HighwirePress.com
UniProt: View information about fluorofix buffer on UniProt.org
Keywords: FluoroFix™ Buffer, Solution, Fixative, Paraformaldehyde, Immunology, Antibodies
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