The buffer solution should be stored at Room Temperature (RT).
Application:
ICC, ICFC
Recommended Usage:
For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website. Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
Application Notes:
This 1X solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.
Application References:
1. Kang, Y.J., et al., 2007. Nature Immunol. 8:601. 2. Kenna. TJ., et al. 2010. J. Immunol. 184:598. PubMed.
Description:
Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.
Other Names:
Fixative, Paraformaldehyde
Antigen References:
1. Current Protocols in Immunology (John Wiley & Sons, New York), Unit 6.24, Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin, NIAID, NIH, Bethesda, MD). 2. Sander, B., et al., 1991. Immunol. Rev. 119:65. 3. Sander, B., et al., 1993. J. Immunol. Meth. 166:201. 4. Prussin, C. et al., 1995. J. Immunol. Meth. 188:117.
