For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website. Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
Application Notes:
This 1X solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.
Application References:
1. Kang, Y.J., et al., 2007. Nature Immunol. 8:601. 2. Kenna. TJ., et al. 2010. J. Immunol. 184:598. PubMed.
Description:
Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.
Other Names:
Fixative, Paraformaldehyde
Antigen References:
1. Current Protocols in Immunology (John Wiley & Sons, New York), Unit 6.24, Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin, NIAID, NIH, Bethesda, MD). 2. Sander, B., et al., 1991. Immunol. Rev. 119:65. 3. Sander, B., et al., 1993. J. Immunol. Meth. 166:201. 4. Prussin, C. et al., 1995. J. Immunol. Meth. 188:117.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
