The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
ICFC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Szabo, S,J., et al. 2000. Cell 100:655 (ICFC, WB) 2. Hwang, E.S., et al. 2005. J Exp Med 202:1289 (ICFC, WB, IP)
Human peripheral blood lymphocytes intracellularly stained with 4B10 FITC (top) or mouse IgG1,k isotype control (bottom) and CD3 APC
Description:
T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
Other Names:
T box expressed in T cells, T box 21, TBLYM
Structure:
T-box transcription factor, approximately 58 kD
Distribution:
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function:
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Antigen References:
1. Szabo, S.J., et al. 2000. Cell 100:655. 2. Szabo, S.J., et al. 2002. Science 295:338. 3. Finotto, S., et al. 2002. Science 295:336. 4. Mullen, A.C., et al. 2001. Science 292:1907.
