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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Description:
SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.
Other Names:
Lymphocyte cytoplasmic protein 2 (LCP2), SH2 domain containing leukocyte 76kD protein
Structure:
Phosphoprotein 76 kD containing SH2 domain in the C-terminus
Distribution:
Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung.
Function:
Adaptor molecule involved in B cell receptor and T cell receptor signaling
Modification:
Phosphorylation (Y113, Y128, Y145, Y423, Y426)
Interaction:
Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2
Antigen References:
1. Jackman KJ, et al.. 1995. J. Biol. Chem. 270:7029. 2. Wardenberg JB, et al.. 1996. J. Biol. Chem. 271:19641. 3. Tuosto L, et al.. 1996. J. Exp. Med. 184:1161. 4. Motto DG, et al.. 1996. J. Exp. Med. 183:1937.
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