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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, immunohistochemical staining1,2 of acetone-fixed frozen sections and Zinc-fixed paraffin-embeded sections, in vitro blocking of allogeneic responses1, Macrophage stimulation3,4 and in vivo depletion.
Application References:
1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP IHC WB) 2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. (IHC) 3. Hirji N, et al. 1997. J. Immunol. 158:1833. 4. Hirji N, et al. 1998. J. Immunol. 160:6004.
CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Other Names:
CD8, Lyt3
Structure:
Ig superfamily, CD8β chain
Distribution:
Most thymocytes, subset of mature T cells (i.e. including most T suppressor/cytotoxic cells), macrophages
Function:
Co-receptor for TCR
Ligand Receptor:
MHC class I molecule
Antigen References:
1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. 2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. 3. Hirji N, et al. 1997. J. Immunol. 158:1833. 4. Hirji N, et al. 1998. J. Immunol. 160:6004.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-rat CD8b
LOU rat splenocytes stained with 341 FITC
Purified anti-rat CD8b
LOU rat splenocytes stained with purified 341, followed by anti-mouse IgG FITC
Alexa Fluor® 488 anti-rat CD8b
LOU rat splenocytes stained with 341 Alexa Fluor® 488
Alexa Fluor® 647 anti-rat CD8b
LOU rat splenocytes stained with 341 Alexa Fluor® 647
