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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications include: immunoprecipitation1, in vitro activation, in vitro inhibition1 of binding of VCAM-1 and mast cells to fibronectin , and in vivo inhibition of VLA-4 functions1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 200106).
Application References:
1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP, Block)
Lou rat blood lymphocytes stained
with MRα4-1 FITC
CD49d is a 150 kD glycoprotein, also known as integrin α4 or VLA-4 α chain. It is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymocytes, and monocytes, while the α4β7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes. The α4β1 and α4β7 integrins mediate cell-cell and cell-matrix interactions by interacting with the ligands CD106 (VCAM-1), MAdCAM-1, and fibronectin. Soluble MRα4-1 antibody can partially inhibit in vitro binding of VCAM-1 and mast cells to fibronectin and inhibit mast cell degranulation. Immobilized MRα4-1 antibody can enhance mast cell degranulation in vitro.
Other Names:
Integrin α4 chain, α4 integrin, VLA-4 α chain
Structure:
Integrin α chain, 150 kD
Distribution:
α4/β1- peripheral T and B lymphocytes, thymocytes, and monocytes; α4/β7- peripheral lymphocytes
Function:
Adhesion
Ligand Receptor:
CD106 (VCAM-1), MAdCAM-1, fibronectin
Antigen References:
1. Yasuda, M., et al. 1995. Int. Immunol. 7:251. 2. Springer, T.A. 1994. Cell 76:301. 3. Issekutz, T. B., et al. 1991. J. Immunol. 147:4178. 4. Issekutz, T. B., et al. 1991. J. Immunol. 147:109. 5. Yang, H., et al. 1995. Transplantation 60:71. 6. Palecanda, A., et al. 1997. J. Immunol. 158:2904.
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FITC anti-rat CD49d
Lou rat blood lymphocytes stained
with MRα4-1 FITC
LEAF™ Purified anti-rat CD49d
LOU rat splenocytes stained with LEAF™ purified MRα4-1, followed by anti-mouse IgG FITC
