Antibodies
|
Search   
FITC anti-rat CD103 (αE Integrin) Antibody
FITC anti-rat CD103 (αE Integrin) Antibody
205505 100 µg $130.00 Qty:
    

Product Details

 
Clone: OX-62 (See other available formats)
Isotype: Mouse IgG1
Isotype Control:FITC Mouse IgG1, κ Isotype Ctrl
Reactivity: Rat
Immunogen: Density gradient enriched lymph veiled cells from PVG rats
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.

Application
References:

  Publication Library
FITC anti-rat CD103 (αE Integrin) Antibody OX-62 <OX62> Image 1
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and CD103 (clone OX-62) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).

FITC anti-rat CD103 (αE Integrin) Antibody OX-62 <OX62> Image 2


Compare all formats

See FITC spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD103, also known as Integrin αE or integrin αIEL, is a single pass transmembrane protein composed by two chains, heavy and light, linked by a disulfide bond. It is expressed in the cell surface as an αE/β7 heterodimer. CD103 mediates adhesion of intraepithelial T cells (IEL) to the epithelium. CD103 is expressed by IEL, effector and memory Tregs, and dendritic cells and their immediate precursors. E-cadherin is the ligand for CD103.

Other Names: Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae
Structure: Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
Distribution: Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
Function: Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
Ligand Receptor: E-cadherin
Antigen
References:

1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.

GeneID: 83577
Latest Publications: View the latest CD103 articles on HighwirePress.com
UniProt: View information about CD103 on UniProt.org
Keywords: FITC anti-rat CD103 (αE Integrin), OX-62 , FITC, Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae , Rat, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
- There are currently no reviews for this product. Be the first to review this product to qualify for a $100 Amazon gift card drawing. -
See more reviews using the Reviews Library


Product FAQs

 
There are no FAQs for this product. For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
FITC Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICFC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-rat CD103 (αE Integrin)OX-62 FC
Alexa Fluor® 647 anti-rat CD103 (αE Integrin)OX-62 FC
Biotin anti-rat CD103 (αE Integrin)OX-62 FC, ICC, IHC, WB


Related Pathways

Pathway
Dendritic Cells
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Rat Immunology > Adhesion Molecules > CD103 (αE Integrin)
Immunology > Rat Immunology > CD and Related Molecules > CD103 (αE Integrin)
Immunology > Rat Immunology > T Regulatory Cells (TReg) > CD103 (αE Integrin)
Immunology > Rat Immunology > Monocyte > CD103 (αE Integrin)
Immunology > Rat Immunology > Innate Immunity > CD103 (αE Integrin)
Immunology > Rat Immunology > Dendritic cells > CD103 (αE Integrin)
Cell Biology > Cell Adhesion > CD103 (αE Integrin)
Immunology > Rat Immunology > CD and Related Molecules > CD103 (αE Integrin) > OX-62

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Alexa Fluor® 488 anti-rat CD103 (αE Integrin)
    LOU rat intestinal intraepithelial lymphocytes

    LOU rat intestinal intraepithelial lymphocytes were stained with CD103 (clone OX-62) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom) and CD3 Alexa Fluor® 647.





  • FITC anti-rat CD103 (αE Integrin)
    LOU rat intestinal intraepithelial lymphocytes

    LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and CD103 (clone OX-62) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).





  • Alexa Fluor® 647 anti-rat CD103 (αE Integrin)
    LOU rat intestinal intraepithelial lymphocytes

    LOU rat intestinal intraepithelial lymphocytes were stained with CD3 FITC and CD103 (clone OX-62) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).





  • Biotin anti-rat CD103 (αE Integrin)
    LOU rat intestinal intraepithelial lymphocytes

    LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and biotinylated CD103 (clone OX-62, top) or mouse IgG1, κ isotype control (bottom), followed by SAV-PE.





x
Copyright © 2014 BioLegend, Inc. All rights reserved.