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For immunofluorescent staining applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Huang F,et al.2007.J. Immunol.179:6504.PubMed 2. Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed
Hela cell was stained by FITC-conjugated anti-NF-κB p50 antibody (clone 4D1). The nucleus is stained by DAPI.
NF-κB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p50 is bound (with p65) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF-κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-κB p50 and has been shown to be useful for Western blotting.
Other Names:
NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1
Structure:
Member of the Rel/dorsal family. One subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits derived from larger precursor proteins
Distribution:
Ubiquitously expressed nuclear protein
Function:
Transcriptional activator, originally identified as an activator of kappa light chain in B cells
Regulation:
Bound (with p65) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB goes to the nucleus to activate gene expression. Stimulation with LPS, TNF-α, phorbol ester, or IL-1 activates
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Purified anti-NF-κB p50
MOLT4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-NF-κB p50 antibody (clone 4D1). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Alexa Fluor® 488 anti-NF-κB p50
Hela cells were stained with Alexa Fluor® 488 anti-NF-kB p50 (4D1) mouse mAb and examined on a fluorescent scope.
FITC anti-NF-κB p50
Hela cell was stained by FITC-conjugated anti-NF-κB p50 antibody (clone 4D1). The nucleus is stained by DAPI.
