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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Blue Laser (488 nm)
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4 and in vivo depletion3-5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 107506).
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro, and to deplete peripheral TCR-γ/δ-bearing T cells in vivo.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse TCR γ/δ
C57BL/6 splenocytes stained with CD3 (145-2C11) APC and UC7-13D5 FITC
Purified anti-mouse TCR γ/δ
C57BL/6 splenocytes stained with 17A2 (CD3) APC and purified UC7-13D5, followed by anti-Armenian hamster IgG PE
PE anti-mouse TCR γ/δ
C57BL/6 mouse lymph node cells stained with 145-2C11 APC and UC7-13D5 PE
Alexa Fluor® 488 anti-mouse TCR γ/δ
C57BL/6 mouse splenocytes stained with CD3 (17A2) PE and UC7-13D5 Alexa Fluor® 488