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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.
Application References:
1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP) 2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC) 3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete) 4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete) 5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete) 6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC) 7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed 8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed 9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
C57BL/6 lymph node cells stained with GL3 FITC and CD3 (145-2C11) APC
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.
Other Names:
T cell receptor gamma/delta
Structure:
Ig superfamily, associates with CD3 complex
Distribution:
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
Function:
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand Receptor:
Some bacterial or tumor antigens bound to MHC class I.
Antigen References:
1. Skarstein K, et al. 1995. Immunology 81:497. 2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167. 3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140. 4. Brandes M, et al. 2005. Science 309:264.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Brilliant Violet 421™ anti-mouse TCR γ/δ
C57BL/6 mouse splenocytes were stained with CD3 APC and TCR γ/δ (clone GL3) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
Biotin anti-mouse TCR γ/δ
C57BL/6 lymph node cells stained with biotinylated GL3 and CD3 (145-2C11) APC, followed by Sav-PE
FITC anti-mouse TCR γ/δ
C57BL/6 lymph node cells stained with GL3 FITC and CD3 (145-2C11) APC
PE anti-mouse TCR γ/δ
C57BL/6 splenocytes stained with CD3 (145-2C11) APC and GL3 PE
APC Anti-mouse TCR γ/δ
C57BL/6 mouse lymph node cells stained with CD3 (145-2C11) PE and GL-3 APC
C57BL/6 mouse lymph node cells stained with CD3 (145-2C11) PE and Armenian hamster IgG APC isotype control
PerCP/Cy5.5 anti-mouse TCR γ/δ
C57BL/6 splenocytes stained with CD3e (145-2C11) FITC and GL3 PerCP/Cy5.5
C57BL/6 splenocytes stained with CD3e (145-2C11) FITC and Armenian Hamster IgG PerCP/Cy5.5 isotype control
PE/Cy7 anti-mouse TCR γ/δ
C57BL/6 mouse splenocytes were stained with CD3 APC and TCR γ/δ (clone GL3) PE/Cy7 (top) or Armenian hamster IgG PE/Cy7 isotype control (bottom).
