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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported (for the relevant formats) applications include: blocking1,2,4,5 and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 115710).
Application References:
1. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. (Block) 2. Ho EL, et al. 2002. J. Immunol. 169:3667. (Block) 3. Maeda M, et al. 2004. J. Immunol. 172:6115. (FC) 4. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (Block) 5. O'Leary JG, et al. 2006. Nature Immunology 7:507. (Block) 6. Tang X, et al. 2006. J. Immunol. 177:7645. (FC)
C57BL/6 splenocytes stained with
C7 PE and NK1.1 FITC
NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8+ T cells, γ/δ T cells and NK1.1+ T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-γ, GM-CSF, TNF-α, MIP1β and others) and granzyme release from NK cells. The C7 antibody has been reported to block NK cell killing of targets expressing NKG2D ligands and induce redirected lysis.
Other Names:
CD314
Structure:
C-type lectin
Distribution:
NK and NK-T cells
Function:
NK cytolytic killing of target cells expressing NGK2D ligands, costimulation NK cells
Ligand Receptor:
Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family
Antigen References:
1. Vance RE, et al. 1999. J. Exp. Med. 190:1801. 2. Vance RE, et al. 1998. J. Exp. Med. 188:1841. 3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755. 4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298. 5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. 6. Ho EL, et al. 2002. J. Immunol. 169:3667.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD314 (NKG2D)
C57BL/6 splenocytes stained with
C7 PE and NK1.1 FITC
PE anti-mouse CD314 (NKG2D)
C57BL/6 splenocytes stained with
C7 PE and NK1.1 FITC
Purified anti-mouse CD314 (NKG2D)
C57BL/6 splenocytes stained with
C7 PE and NK1.1 FITC
LEAF™ Purified anti-mouse CD314 (NKG2D)
C57BL/6 splenocytes stained with
C7 PE and NK1.1 FITC
FITC anti-mouse CD314 (NKG2D)
C57BL/6 splenocytes stained with
C7 PE and NK1.1 FITC
