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FITC anti-mouse Ly49D Antibody
FITC anti-mouse Ly49D Antibody
138303 50 µg $95.00 Qty:
    

Product Details

 
Clone: 4E5 (See other available formats)
Isotype: Rat IgG2a, κ
Isotype Control:FITC Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.

Application
References:

  Publication Library
FITC anti-mouse Ly49D Antibody 4E5 Image 1
C57BL/6 splenocytes stained with CD49b (DX5) APC and 4E5 FITC

FITC anti-mouse Ly49D Antibody 4E5 Image 2
C57BL/6 splenocytes stained with CD49b (DX5) APC and rat IgG2a FITC isotype control


Compare all formats

See FITC spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.  Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J.  The receptors form homodimers that bind to MHC class I alloantigens.  Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif.  The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-Dd, Dr and Dsp2, but not with H2b or H2k class I antigens.  Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells.  In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V.

Other Names: Ly-49D
Structure: A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Distribution: Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.
Function: Plays an important role in activating natural killer (NK) cell cytolytic activity.
Ligand Receptor: MHC class I alloantigens
Antigen
References:

1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.

GeneID: 16635
Latest Publications: View the latest Ly49D articles on HighwirePress.com
UniProt: View information about Ly49D on UniProt.org
Keywords: FITC anti-mouse Ly49D, 4E5, FITC, Ly-49D , Mouse, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
BioLegend 138304 (FC) /4 2012-07-19
      See more reviews using the Reviews Library


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DescriptionCloneApplications
FITC Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
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Other Formats

DescriptionCloneApplications
Alexa Fluor® 647 anti-mouse Ly49D4E5FC
PE anti-mouse Ly49D4E5FC
Purified anti-mouse Ly49D4E5FC, IP


Related Pathways

Pathway


Related Categories

Category
Immunology > Mouse Immunology > CD and Related Molecules > Ly49D > 4E5
Immunology > Mouse Immunology > Innate Immunity > Ly49D
Immunology > Mouse Immunology > NK/NKT Cells > Ly49D

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse Ly49D
    C57BL/6 splenocytes stained with CD49b

    C57BL/6 splenocytes stained with CD49b (DX5) APC and purified 4E5 conjugated with FITC

    C57BL/6 splenocytes stained with CD49b

    C57BL/6 splenocytes stained with CD49b (DX5) APC and rat IgG2a FITC isotype control

  • FITC anti-mouse Ly49D
    C57BL/6 splenocytes stained with CD49b

    C57BL/6 splenocytes stained with CD49b (DX5) APC and 4E5 FITC

    C57BL/6 splenocytes stained with CD49b

    C57BL/6 splenocytes stained with CD49b (DX5) APC and rat IgG2a FITC isotype control

  • Alexa Fluor® 647 anti-mouse Ly49D
    C57BL/6 splenocytes stained with CD49b

    C57BL/6 splenocytes stained with CD49b (DX5) PE and 4E5 Alexa Fluor® 647

    C57BL/6 splenocytes stained with CD49b

    C57BL/6 splenocytes stained with CD49b (DX5) PE and rat IgG2a Alexa Fluor® 647

  • PE anti-mouse Ly49D
    C57BL/6 splenocytes stained with NK1.1

    C57BL/6 splenocytes stained with NK1.1 (PK136) Alexa Fluor® 647 and 4E5 PE (top) or rat IgG2a PE isotype control (bottom)





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